Biophysical mechanisms of stem cell regulation

干细胞调节的生物物理机制

基本信息

批准号：
7339899
负责人：
Nestor J Oviedo
金额：
$ 5.13万
依托单位：
ADA FORSYTH INSTITUTE, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-01-01 至 2008-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7339899
关键词：
Address Adult Area Attention Behavior Biochemical Biological Biological Markers Biological Models Cell Cycle Cells Cellular biology Communication Complement Complex Computer information processing Cues Daily Data Developmental Biology Elements Embryo Event Future Genes Genomics Goals Homeostasis Human body Injury Invertebrates Ion Channel Ions Malignant Neoplasms Mediating Modeling Molecular Molecular Genetics Morphogenesis Morphology Natural regeneration Organism Phenotype Physiological Planarians Platyhelminths Population Process Proliferating Publications Pump RNA Interference Range Regulation Research Retinal Cone Role Scientist Signal Transduction Source Staging Stem cells Systems Biology Testing Tissues Training Transcript Undifferentiated Validation Work adult stem cell base cell behavior cell growth regulation design experience insight loss of function migration novel pH gradient research study restoration senescence tissue regeneration voltage

项目摘要

The interplay between undifferentiated and differentiated tissue allows the fine regulation of body homeostasis. Much is unknown about the molecular events underlying this process, but it is likely to involved adult stem cell activation. In addition to homeostasis, stem cells may be harnessed to induce the regeneration of tissue damaged by senescence, cancer, or injury. Such biomedical approaches require a basic molecular understanding of stem cells in their natural behavior. In complement to the current focus on biochemical signals, we will explore an important but largely mysterious set of cellular control parameters: ion flows. The main hypothesis that will be tested in this proposal is that stem cells are controlled in part by biophysical signals mediated by gap junctional communication and the activity of ion channels and pumps. Capitalizing on a powerful model system, the planarian, I propose to gain molecular, mechanistic insight into the novel and important role of endogenous physiological signals regulating the interactions between adult stem cells and differentiating tissue during physiological turnover.and regeneration.

未分化组织和分化组织之间的相互作用允许身体的精细调节体内平衡。关于这一过程背后的分子事件还有很多未知之处，但它很可能涉及成体干细胞激活。除了体内平衡外，干细胞还可用于诱导因衰老、癌症或损伤而受损的组织的再生。这种生物医学方法需要对干细胞自然行为的基本分子理解。作为对当前重点的补充生化信号，我们将探索一组重要但很大程度上神秘的细胞控制参数：离子流。该提案将检验的主要假设是干细胞部分受控于由间隙连接通讯以及离子通道和泵的活动介导的生物物理信号。利用涡虫这个强大的模型系统，我建议获得分子、机制的洞察力内源性生理信号调节成人之间相互作用的新颖而重要的作用生理更新和再生过程中的干细胞和分化组织。