Power Tower: A Novel Paradigm for Exercsie Training in Drosophila melanogaster

动力塔：果蝇运动训练的新范式

基本信息

批准号：
8110334
负责人：
Robert John Wessells
金额：
$ 21.52万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2013-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8110334
关键词：
Age Aging Animal Model Area Biogenesis Biological Models Candidate Disease Gene Cardiac Cardiac Output Data Set Drosophila genus Drosophila melanogaster Exercise Exercise Physiology Future Genes Genetic Genetic Models Genotype Grouping Health Human Intervention Invertebrates Learning Mediator of activation protein Meta-Analysis Mind Mission Mitochondria Modeling Muscle Muscle function Myopathy National Heart, Lung, and Blood Institute Organism Orthologous Gene Oxidative Stress Pathway interactions Pattern Performance Physiological Physiology Prevention program Property Protocols documentation Reporting Research Resistance Sequence Homology Stress System Testing Time Training Training Programs Triglycerides United States National Institutes of Health Ursidae Family Vertebrates age related anti aging base design experience fly improved loss of function mutation mimetics novel overexpression programs protein function research study response therapy design

项目摘要

DESCRIPTION (provided by applicant): Endurance exercise has emerged in recent years as a potent intervention capable of improving a wide variety of health parameters in humans and in vertebrate models. Although much has been learned about physiological responses to exercise training in vertebrates, the genetic components necessary for these responses are still not well understood. With this in mind, we have developed a novel endurance exercise model in a genetically tractable research organism, the fruit fly. Flies of multiple genotypes respond to a program of endurance exercise by improving climbing ability and cardiac stress resistance, as well as increasing mitochondrial activity, suggesting that exercise produces similar effects in invertebrates as in vertebrates. In order to fully establish the utility of this model system, however, it will first be necessary to more fully characterize the similarities and differences between the exercise response in flies and in vertebrates. Here, we propose to establish the relevance of flies as an exercise model by characterizing a variety of physiological responses to exercise in the fly model. Secondly, we propose to establish conservation of genetic pathways involved in these responses by testing candidate genes known to be important in regulating vertebrate physiology for their effects on the exercise response of the fly. Once the relevance of flies as an exercise model is established, the power of fly genetics can be used to uncover novel conserved genetic factors capable of regulating or mimicking the exercise response in vertebrates. PUBLIC HEALTH RELEVANCE: Understanding genetic mechanisms of exercise physiology is relevant to the mission of the National Institutes of Health and the National Heart Lung and Blood Institute because the identification of key genetic components of the response will allow design of treatments and prevention programs for degenerative muscular diseases and age-related decline in muscular function.

描述（由申请人提供）：近年来，耐力运动作为一种有效的干预措施出现，能够改善人类和脊椎动物模型的各种健康参数。尽管人们对脊椎动物运动训练的生理反应已经了解很多，但这些反应所必需的遗传成分仍不清楚。考虑到这一点，我们在基因可控的研究生物——果蝇中开发了一种新颖的耐力运动模型。多种基因型的果蝇通过提高攀爬能力和心脏应激抵抗力以及增加线粒体活性来对耐力运动计划做出反应，这表明运动对无脊椎动物和脊椎动物产生相似的效果。然而，为了充分建立该模型系统的实用性，首先有必要更全面地描述果蝇和脊椎动物运动反应之间的相似性和差异。在这里，我们建议通过表征果蝇模型中运动的各种生理反应来建立果蝇作为运动模型的相关性。其次，我们建议通过测试已知对调节脊椎动物生理学重要的候选基因及其对果蝇运动反应的影响来建立参与这些反应的遗传途径的保护。一旦建立了果蝇作为运动模型的相关性，果蝇遗传学的力量就可以用来发现能够调节或模仿脊椎动物运动反应的新的保守遗传因子。公共健康相关性：了解运动生理学的遗传机制与美国国立卫生研究院和国家心肺和血液研究所的使命相关，因为识别反应的关键遗传成分将有助于设计退行性肌肉萎缩症的治疗和预防计划。疾病和与年龄相关的肌肉功能下降。