Live-cell wavefront metrology

活细胞波前测量

• 批准号: ST/F001754/1
• 负责人: Alan Howard Greenaway
• 金额: $ 31.72万
• 依托单位: Heriot-Watt University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=ST%2FF001754%2F1
• 关键词: Live; cell; wavefront; metrology

The study of living organisms is increasingly based on studies of the dynamics of processes occurring within the living organism rather than, as in the past, the study of dead, stained, dehydrated and sectioned secimens. Wavefront sensing techniques used to measure the effects of atmospheric turbulence on high-precision imaging in astronomy provide a mechanism for the tracking of the fluorescent particles frequenlty used as tracers in live-cell imaging. This biology application has much in common with astronomy - the light levels available are low (stars are faint, bright fluorescent particles poisen the cells), the observation may be subject to time and space-varying distortions (due to the atmosphere in astronomy, due to the specimen itself in live-cell imaging), dependent on the application, different spectral (colour) regions or spectral bands may need to be used. This programme seeks to use wavefront sensing techniques developed for use in astronomy to the problems of imaging 3-dimensional and time-varying processes in biology. Many developmental studies of living specimens will benefit from a successful application of these techniques.

对活生物体的研究越来越多地基于对活生物体内部发生的过程动力学的研究，而不是像过去那样对死亡、染色、脱水和切片的标本进行研究。用于测量大气湍流对天文学高精度成像影响的波前传感技术提供了一种跟踪活细胞成像中常用作示踪剂的荧光粒子的机制。这种生物学应用与天文学有很多共同点 - 可用的光水平较低（恒星微弱，明亮的荧光粒子使细胞中毒），观察可能会受到时间和空间变化的扭曲（由于天文学中的大气，由于活细胞成像中的样本本身），根据应用，可能需要使用不同的光谱（颜色）区域或光谱带。该项目旨在利用为天文学开发的波前传感技术来解决生物学中的 3 维和时变过程成像问题。许多活体标本的发育研究将受益于这些技术的成功应用。