Probiotic Metabolism of Rice Bran Phytochemicals for Chemoprevention

米糠植物化学物质的益生菌代谢用于化学预防

基本信息

批准号：
8053395
负责人：
Elizabeth P Ryan
金额：
$ 7.13万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-29 至 2012-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8053395
关键词：
Address Affect Africa Antioxidants Attention Bifidobacterium Biological Availability Biological Models Blood Calories Canis familiaris Carbohydrates Chemoprevention Chemopreventive Agent Clinical Research Companions Complex Conflict (Psychology)Consumption Coupled Data Detection Development Dietary Factors Dietary Fiber Dietary Intervention Dietary intake Digestion Disease Resistance Dose Epidemiology Escherichia Escherichia coli Essential Fatty Acids Exhibits Experimental Models Family Fermentation Fiber Food Foundations Gas-Liquid Chromatography Gene Expression Hematopoietic Neoplasms Humanities India Intake Investigation Knowledge Lactobacillus Lymphocyte Lymphoma Malignant Neoplasms Mass Spectrum Analysis Measures Metabolism Methods Microbiology Micronutrients Minerals Modeling Newly Diagnosed Non-Hodgkin&apos s Lymphoma Nutritional Organism Oryza sativa Pathology Patients Philippines Phospholipids Phytic Acid Phytochemical Plant Diseases Plants Play Prevention Probiotics Property Prospective Studies Proteins Publishing Reporting Research Research Personnel Review Literature Rice Risk Rodent Role Solid Source Techniques Testing Tissues Toxicology Variant Vitamins bioactive food component cancer prevention cancer risk catalyst cofactor comparative feeding genome sequencing human disease in vivo lymph nodes novel oncology pre-clinical stoichiometry trait uptake

项目摘要

DESCRIPTION (provided by applicant): Although epidemiologic evidence supports an inverse association between whole grain consumption and cancer risk, results are inconclusive as to the precise bioactive food components by which chemoprevention occurs. Emerging data has identified phytochemicals contained in rice bran that may reduce cancer risk; however, little is known about the concentrations and bioavailability of these chemopreventive phytochemicals in existing varieties and landraces of cultivated rice. Rice (Oryza sativa) is the primary source of dietary calories for half of humanity and is the first crop plant for which a high-quality reference genome sequence has been produced. Rice varieties that capture the impressive genotypic and phenotypic diversity of domesticated rice world-wide should be investigated for differential cancer prevention properties. Moreover, an extensive review of the literature revealed limited research aimed at exploring the role probiotics play in metabolizing rice bran. Thus, the development of an experimental model to assess the effects of rice bran-probiotic interactions on bioavailability of chemopreventive compounds to blood, lymphocytes and lymph nodes is proposed. Findings from the studies proposed herein will fill a significant gap in our knowledge regarding rice bran phytochemical diversity and bioavailability for chemoprevention. The stoichiometry of selected bioactive rice bran components with reported cancer prevention properties will be determined in three genetically diverse rice cultivars. These rice varieties will be fermented with probiotics, Lactobacillus caseii and Bifidobacteria longum, and one resident non-probiotic, Escherichia coli, in Aim 1 to determine their effect on levels of the selected metabolites. The probiotic digestion of rice bran is hypothesized to enhance bioavailability of rice bran derived chemopreventive compounds. Phytochemicals and metabolites will be detected by liquid and gas chromatography coupled with -mass spectrometry (LC-MS, GC-MS). A second aim is proposed to determine the dose of rice bran that achieves greatest bioavailability of target phytochemicals and metabolites into blood, lymphocytes and lymph nodes of naturally occurring canine lymphoma patients. This comparative oncology model is novel for assessing bioavailability of dietary rice bran components. Furthermore, it has been well established that naturally developing canine cancer exhibits greater translational potential compared to experimentally induced cancer in rodents, particularly for lymphoma. The pilot dose determination studies are feasible for testing the hypothesis that bioavailability of cancer prevention compounds from dietary whole rice bran intake varies across genetically diverse rice cultivars and by probiotic fermentation. Indeed, pre-clinical and clinical studies that evaluate the role of fiber or isolated rice bran compounds and cancer risk are conflicting, and we put forth that novel transdisciplinary scientific approaches are necessary to advance the nutritional investigation of rice bran for cancer prevention.

描述（由申请人提供）：尽管流行病学证据支持全谷物消耗与癌症风险之间的反比相关性，但结果对于发生化学预防的确切生物活性食品成分尚无定论。新兴数据已经确定了水稻麸皮中包含的植物化学物质，这些化学物质可能会降低癌症的风险。然而，对于这些化学预防植物化学物质在现有品种和栽培大米的陆地中的浓度和生物利用度知之甚少。大米（Oryza sativa）是人类一半的饮食卡路里的主要来源，是产生高质量参考基因组序列的第一个作物植物。应研究全世界驯化水稻的令人印象深刻的基因型和表型多样性的水稻品种，以供预防疾病的癌症预防特性。此外，对文献的广泛综述揭示了有限的研究，旨在探索益生菌在代谢大米麸中的作用。因此，提出了一种实验模型，以评估水稻麸皮相互作用对血液，淋巴细胞和淋巴结的化合物对化学化合物的生物利用度的影响。本文提出的研究的结果将填补我们关于米麸植物化学多样性和化学预防的生物利用度的显着空白。将在三种遗传多样化的水稻品种中确定，选定的生物活性水稻麸组成分的化学计量法将确定。这些水稻品种将用益生菌，乳杆菌和双歧杆菌长乳杆菌发酵，以及一种居民的非豆类大肠杆菌，以确定其对所选代谢物水平的影响。假设水稻麸皮的益生菌消化以增强米麸的衍生化学预防化合物的生物利用度。植物化学物质和代谢产物将通过液态和气相色谱与质谱法（LC-MS，GC-MS）检测。提出了第二个目标来确定靶植物植物化学物质和代谢产物最大的生物利用度的剂量，使其成为血液，淋巴细胞和天然犬淋巴瘤患者的淋巴细胞和淋巴结。这种比较肿瘤学模型是用于评估饮食米麸组成分的生物利用度的新颖新颖的。此外，与实验诱导的啮齿动物（尤其是淋巴瘤）相比，自然发展的犬类癌具有更大的翻译潜力。试点剂量测定研究对于测试以下假设，即饮食中饮食中的癌症预防化合物的生物利用度在遗传多样的水稻品种和益生菌发酵过程中各不相同。实际上，评估纤维或孤立的水稻麸皮化合物和癌症风险作用的临床前和临床研究是矛盾的，我们提出，新型的跨学科科学方法对于促进水稻麸皮的营养研究是必要的，以预防癌症。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Metabolomics and metabolic pathway networks from human colorectal cancers, adjacent mucosa, and stool.

DOI：
10.1186/s40170-016-0151-y
发表时间：
2016
期刊：
Cancer & metabolism
影响因子：
5.9
作者：
Brown DG;Rao S;Weir TL;O'Malia J;Bazan M;Brown RJ;Ryan EP
通讯作者：
Ryan EP

Enhanced community structure detection in complex networks with partial background information.

DOI：
10.1038/srep03241
发表时间：
2013-11-19
期刊：
Scientific reports
影响因子：
4.6
作者：
Zhang ZY;Sun KD;Wang SQ
通讯作者：
Wang SQ

Advances in Nutritional Metabolomics.

营养代谢组学的进展。

DOI：
10.2174/2213235x11301020001
发表时间：
2013
期刊：
Current Metabolomics
影响因子：
0
作者：
Ryan,ElizabethP;Heuberger,AdamL;Broeckling,CoreyD;Borresen,EricaC;Tillotson,Cadie;Prenni,JessicaE
通讯作者：
Prenni,JessicaE

The relevance and applicability of oocyst prevalence as a read-out for mosquito feeding assays.

DOI：
10.1038/srep03418
发表时间：
2013-12-04
期刊：
Scientific reports
影响因子：
4.6
作者：
Stone WJ;Eldering M;van Gemert GJ;Lanke KH;Grignard L;van de Vegte-Bolmer MG;Siebelink-Stoter R;Graumans W;Roeffen WF;Drakeley CJ;Sauerwein RW;Bousema T
通讯作者：
Bousema T

Metabolomic and functional genomic analyses reveal varietal differences in bioactive compounds of cooked rice.

代谢组学和功能基因组分析揭示了米饭生物活性化合物的品种差异。

DOI：
10.1371/journal.pone.0012915
发表时间：
2010-09-23
期刊：
PLOS ONE
影响因子：
3.7
作者：
Heuberger, Adam L.;Lewis, Matthew R.;Chen, Ming-Hsuan;Brick, Mark A.;Leach, Jan E.;Ryan, Elizabeth P.
通讯作者：
Ryan, Elizabeth P.