ROS Analytical Core

ROS 分析核心

• 批准号: 8066620
• 负责人: BHUPENDRA S KAPHALIA
• 金额: $ 12.98万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8066620
• 关键词: Cell membrane; Cells; Chemicals; Collaborations; Core Facility; Generations; Histopathology; Image; Life; Lipid Peroxidation; Lipids; Measurement; Measures; Modeling; Oxidative Stress; Pain; Pathogenesis; Peripheral; Phospholipids; Proteins; Reactive Oxygen Species; Role; Source; Time; Unsaturated Fatty Acids; Work; cell fixing; cellular imaging; functional outcomes; in vivo; oxidation; peroxidation

Core B is the ROS Analytical Core. This is a new core that became necessary because all the projects require quantitative measurement of reactive oxygen species (ROS). Measuring ROS is challenging, particularly when done in vivo, because of multiple intracellular sources of ROS in cells and the known propensity of one form of ROS to readily transform into another. One overriding facet of oxidative stress, however, is peroxidation of unsaturated fatty acid moieties of neutral lipids and phospholipids in cell membranes and oxidation of cellular proteins. Moreover, measuring specific ROS may not explain the functional outcomes in pathological pain and related pathogenesis. An assessment of ROS-induced lipid peroxidation and oxidation of proteins appears to be a logical choice for understanding the underlying mechanism in our pain model. The findings of lipid peroxidation and oxidation proteins will be supported by the real-time live cell and histochemical fixed cell imaging to be conducted in collaboration with Core C. Therefore, ROS Analytical Core will measure lipid peroxidation products and oxidized proteins, and ROS generation in live or fixed cells. The ROS Analytical Core is composed of three branches with an assigned Co-Director for each: 1) Lipid Peroxidation Analysis, 2) Protein Oxidation Analysis, and 3) Histopathology branches. While branches 1) and 2) are for the chemical analysis, branch 3) is to measure intracellular ROS levels using both real-time live-cell imaging and histochemical imaging from fixed cells. For the use of the imaging facility, this core works very closely with Core C (Imaging Core) and the Co-Director of Histopathology of this core is also a Co-Director of the Imaging Core. Therefore, the ROS Analytical Core becomes an integral part of the PPG in delineating the role of ROS in central and peripheral sensitization in pain.

核B是ROS分析核心。这是一个新的核心，因为所有项目 需要定量测量活性氧（ROS）。测量ROS具有挑战性， 特别是在体内完成时，由于细胞中ROS的多个细胞内来源和已知的 一种形式的ROS倾向，容易转变为另一种。一个氧化应激的覆盖面， 然而，是细胞中中性脂质和磷脂的不饱和脂肪酸部分的过氧化 膜和细胞蛋白的氧化。此外，测量特定的ROS可能无法解释 病理疼痛和相关发病机理中的功能结果。 ROS诱导的脂质的评估 蛋白质的过氧化和氧化似乎是理解潜在的逻辑选择 我们的疼痛模型中的机制。脂质过氧化和氧化蛋白的发现将得到 与CoreC合作进​​行的实时活细胞和组织化学固定细胞成像 因此，ROS分析核心将测量脂质过氧化产物和氧化蛋白，ROS 在活细胞或固定细胞中产生。 ROS分析核由三个分支组成，分配了 每个：1）脂质过氧化分析，2）蛋白质氧化分析和3）组织病理学 分支。分支1）和2）用于化学分析，分支3）是测量细胞内ROS 使用固定细胞的实时活细胞成像和组织化学成像的水平。用于使用 成像设施，该核心与核心C（成像核心）和联合导演非常紧密地工作 该核心的组织病理学也是成像核心的共同导演。因此，ROS分析核心 在描述ROS在中央和周围敏化中的作用中，成为PPG的组成部分 疼痛。