NEW ENERGY TRANSFER PROBES FOR SINGLE CELL IMAGING

用于单细胞成像的新能量转移探针

• 批准号: 7373134
• 负责人: KEVIN BURGESS
• 金额: $ 1.35万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7373134
• 关键词: NEW; ENERGY; TRANSFER; PROBES; SINGLE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Single molecule studies of protein interactions with artificial and living cell membranes have progressed significantly since the commencement of the grant period. Interactions of proteins with the cell membrane are essential for efficient uptake of macromolecules into living cells. Single molecule fluorescence approaches have allowed us to study primary events such as the binding of macromolecules to membranes, lateral motion in the membrane plane and internalization for individual molecules, with all measurements free from ensemble averaging. Real-time single molecule imaging is the ability to record movies of molecular motions and to follow the spatial pathways and chemical reactions of individual macromolecules or their complexes. Rhodamine based fluorescent cassettes are being synthesized in the research group of Kevin Burgess and will be investigated in our laboratories using single molecule spectroscopic tools. These cassettes were shown to employ large stokes shifts making them suitable to be used in potential applications of single molecule experiments in solution or in single cells, where the weak fluorescence has to be distinguished from scattered excitation light and other background sources. Other possible applications include their use for two-photon imaging of biological samples using a confocal microscope setup.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。自资助期开始以来，蛋白质与人造细胞膜和活细胞膜相互作用的单分子研究取得了显着进展。蛋白质与细胞膜的相互作用对于活细胞有效摄取大分子至关重要。单分子荧光方法使我们能够研究主要事件，例如大分子与膜的结合、膜平面的横向运动和单个分子的内化，所有测量都不受整体平均的影响。实时单分子成像是记录分子运动的电影并跟踪单个大分子或其复合物的空间路径和化学反应的能力。 Kevin Burgess 研究小组正在合成基于罗丹明的荧光盒，并将在我们的实验室中使用单分子光谱工具进行研究。这些盒被证明采用大斯托克斯位移，使其适合用于溶液或单细胞中单分子实验的潜在应用，其中弱荧光必须与散射激发光和其他背景源区分开。其他可能的应用包括使用共焦显微镜装置对生物样品进行双光子成像。