Production, Validation and Distribution of the Xenopus ORFeome

非洲爪蟾 ORFeome 的生产、验证和分发

• 批准号: 8154960
• 负责人: Michael James Gilchrist
• 金额: $ 72.36万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8154960
• 关键词: Animal Model; Biological Assay; Cloning; Communities; Complementary DNA; Data; Destinations; Funding; Genome; Genomics; Human; Investments; Laboratories; Medical center; Open Reading Frames; Output; Pediatric Hospitals; Plasmids; Production; Productivity; Proteins; Rana; Reagent; Research; Research Personnel; Resources; Screening procedure; Series; System; Time; United States National Institutes of Health; Validation; Voting; Xenopus; Xenopus laevis; Yeasts; expression vector; functional genomics; in vivo; meetings; tool; vector

DESCRIPTION (provided by applicant): We propose to generate, validate and distribute a Xenopus ORFeome, which we define as a fully sequenced, validated set of Xenopus cDNA clones containing one each of every open reading frame (ORF) encoded in the genome, in a format in which any ORF sequence can be easily transferred using recombineering into a diverse array of expression vectors. This one set of reagents will greatly decrease the time to characterize any protein in the myriad of functional assays carried out by the entire Xenopus community. But most importantly, an ORFeome set will allow high-throughput in vivo functional-genomic screening in manner currently not feasible, and will take advantage of a particular strength of the Xenopus system. These clones will be made available, without restriction, to researchers worldwide. The Xenopus community is fully supportive of this project and at a recent PI meeting (Lake Louise, September 2010) the ORFeome was voted as the Top priority of needed Xenopus resources. PUBLIC HEALTH RELEVANCE: We will develop a genomic tool that will greatly enhance the scientific output of the entire scientific community that utilizes the frog Xenopus as a model organism, known as the ORFeome. This will greatly enhance the return of the investment of NIH dollars into Xenopus research which currently contains over 100 laboratories and represents ~$124,000,000 of NIH funding per year. An ORFeome is a highly validated tool that has successfully increased the productivity of human, worm and yeast laboratories. The Xenopus community is fully supportive of this project and at a recent PI meeting (Lake Louise, September 2010) the ORFeome was voted as the Top priority of needed Xenopus resources. Moreover the production of the ORFeome will greatly enhance the usefulness of the current genome information, which is the second highest priority of the community.

描述（由申请人提供）：我们建议生成，验证和分发Xenopus orfeome，我们将其定义为经过完整的测序，经过验证的，经过每个开放阅读框架（ORF）中的一个经过验证的Xenopus cDNA克隆集，以基因组中的每个开放阅读框架（ORF）中的一个，以这种形式可以轻松地将任何ORF序列转移到Recombineering中。这套试剂将大大减少表征整个Xenopus社区进行的无数功能测定中的任何蛋白质的时间。但最重要的是，Orfeome集将允许以当前不可行的方式进行体内功能基因组筛查，并利用Xenopus系统的特殊强度。这些克隆将不受限制地向全球研究人员提供。 Xenopus社区完全支持该项目，在最近一次的PI会议（Louise，2010年9月）中，Orfeome被评为需要的Xenopus Resources的首要任务。 公共卫生相关性：我们将开发一种基因组工具，该工具将极大地增强整个科学界的科学输出，该工具将青蛙Xenopus用作模型生物，称为Orfeome。这将大大提高NIH美元投资到Xenopus研究的回报，该研究目前包含100多个实验室，每年约为NIH资金约12.4万美元。 Orfeome是一种高度验证的工具，已成功提高了人类，蠕虫和酵母实验室的生产率。 Xenopus社区完全支持该项目，在最近一次的PI会议（Louise，2010年9月）中，Orfeome被评为需要的Xenopus Resources的首要任务。此外，Orfeome的生产将大大提高当前基因组信息的有用性，这是社区第二高的优先事项。