Confocal Microscope Zeiss LSM710

共焦显微镜蔡司 LSM710

• 批准号: 7794527
• 负责人: HORST B FISCHER
• 金额: $ 49.46万
• 依托单位: CHILDREN'S HOSPITAL & RES CTR AT OAKLAND
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-22 至 2011-04-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794527
• 关键词: Area; Automobile Driving; Biology; Cancer Biology; Carbon Dioxide; Cells; Chlamydia; Complement; Consultations; Fee-for-Service Plans; Funding; Image; Institution; Life; Lipids; Lipoproteins; Meningitis; Metabolism; Micronutrients; Microscope; Microscopy; Nature; Pathogenesis; Pediatric Hospitals; Physiological; Request for Proposals; Research; Research Institute; Research Personnel; Signal Transduction; Site; Skin Cancer; Sphingosine; Staging; Structure; System; Temperature; Time; base; cellular imaging; cystic fibrosis airway; detector; experience; instrument; research study; vaccine development

DESCRIPTION (provided by applicant): This proposal requests support to purchase a Zeiss LSM 710 Spectral Confocal Microscope for use at Children's Hospital Oakland Research Institute (CHORI) to facilitate an array of projects that mainly involve microscopy of live cells. Nine major users and a total of 16 NIH-funded projects at CHORI will immediately benefit from this instrument. The requested instrument is configured to support cellular localization of signals and their physiological functions in live cells. This will be made possible by environmental controls (temperature, CO2) on the stage of the microscope, continuous time-lapse recording capabilities, and a 3-channel spectral detector including ratio imaging capabilities. There is no confocal microscope at CHORI and no other high-end microscope. Currently, projects requiring microscopy are done off-site, mainly at the microscopy facilities at UC Berkeley, which is located in driving distance from CHORI. These fee-for-service instruments are in high demand, which creates a shortage of the large time blocks needed by CHORI investigators, in particular for live cell imaging. In view of the time-sensitive nature of these experiments and the difficulty of transporting live cells between institutions, the installation of a confocal microscope at CHORI for live cell imaging will enhance an array of ongoing NIH-funded projects, including research in the areas of cystic fibrosis, airway biology, meningitis vaccine development, sphingosine metabolism and cancer biology, pathogenesis of Chlamydia, lipoprotein and lipid biology, and micronutrient research, and skin cancer.. After extensive consultation with external microscopy experts and consideration of the available microscopes, the Zeiss LSM 710 was identified as the optimal system from a number of competing confocal systems by an internal microscopy committee that was founded at CHORI to establish and organize a user base and develop the structures necessary for an institutional microscopy core. The selected system is highly versatile and expandable if the need arises. All identified major users are experienced in using various Zeiss confocal microscopes, and there is microscopy expertise at CHORI to operate and maintain the proposed instrument. The requested Zeiss LSM 710 is one of the most advanced and sensitive instruments in its class and will complement other confocal microscopes available in the area. This microscope will significantly advance the current projects at CHORI and will add to the pool of shared instruments available.

描述（由申请人提供）：该提案请求支持购买蔡司 LSM 710 光谱共焦显微镜，供奥克兰儿童医院研究所 (CHORI) 使用，以促进一系列主要涉及活细胞显微镜检查的项目。 CHORI 的 9 个主要用户和总共 16 个 NIH 资助的项目将立即从该仪器中受益。 所需的仪器被配置为支持信号的细胞定位及其在活细胞中的生理功能。这将通过显微镜载物台上的环境控制（温度、CO2）、连续延时记录功能以及包括比率成像功能的 3 通道光谱探测器来实现。 CHORI 没有共焦显微镜，也没有其他高端显微镜。目前，需要显微镜的项目都是在场外完成的，主要是在距离 CHORI 不远的加州大学伯克利分校的显微镜设施中。这些按服务收费的仪器需求量很大，导致 CHORI 研究人员所需的大量时间出现短缺，特别是在活细胞成像方面。鉴于这些实验的时间敏感性以及在机构之间运输活细胞的困难，在 CHORI 安装共聚焦显微镜进行活细胞成像将加强一系列正在进行的 NIH 资助的项目，包括以下领域的研究囊性纤维化、气道生物学、脑膜炎疫苗开发、鞘氨醇代谢和癌症生物学、衣原体发病机制、脂蛋白和脂质生物学、微量营养素研究和皮肤癌。在与外部显微镜进行广泛咨询后经过专家的评估和对可用显微镜的考虑，蔡司 LSM 710 被 CHORI 成立的内部显微镜委员会从许多竞争共焦系统中确定为最佳系统，该委员会旨在建立和组织用户基础并开发机构所需的结构显微镜核心。如果需要的话，所选系统具有高度通用性和可扩展性。所有确定的主要用户都具有使用各种蔡司共焦显微镜的经验，并且 CHORI 拥有显微镜专业知识来操作和维护拟议的仪器。 所需的蔡司 LSM 710 是同类产品中最先进、最灵敏的仪器之一，将补充该领域的其他共焦显微镜。该显微镜将显着推进 CHORI 当前的项目，并将添加到可用的共享仪器池中。