Spinning Disk Confocal Microscope for the University of California, Berkeley

加州大学伯克利分校转盘共焦显微镜

• 批准号: 7793587
• 负责人: KARSTEN WEIS
• 金额: $ 49.85万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-13 至 2011-05-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793587
• 关键词: California; Cell physiology; Cells; Cellular biology; Cytoskeleton; Development; Developmental Process; Electrons; Ensure; Funding; Image; Imaging Device; Infection; Institutes; Laboratories; Lasers; Life; Lighting; Maintenance; Membrane Protein Traffic; Microscope; Microscopy; Molecular; Morphogenesis; Neurosciences; Real-Time Systems; Research; Sampling; Scanning; System; Time; Tissues; Training; Training Support; United States National Institutes of Health; Universities; anticancer research; base; charge coupled device camera; instrument; molecular imaging; nucleocytoplasmic transport; operation; pathogen

DESCRIPTION (provided by applicant): A group of six NIH-funded cell and developmental biologists from the University of California Berkeley, together with the UC Berkeley Molecular Imaging Center (MIC), request funds to purchase a spinning disk confocal microscope system for real-time and time-lapse imaging of dynamic cellular and developmental processes. Spinning disk confocal microscopes have emerged as unique and powerful tools for imaging dynamic cellular and developmental processes including cytoskeleton dynamics, membrane trafficking, nuclear transport, pathogen infection, and tissue morphogenesis. These instruments employ a parallel, multi-beam scanning approach that allows significantly faster image acquisition compared to standard laser scanning confocal microscopes, enabling the imaging of cellular processes that occur on a very rapid time scale. Moreover, when combined with very sensitive, electron multiplying CCD cameras, such systems have the additional advantage of acquiring images at low illumination levels, which minimizes photodamage to samples and permits extended imaging of living cells and tissues. Although access to a spinning disk microscope has become vital for the six major users on this application to accomplish the objectives of their NIH funded research, no such system is currently available for their use on the UC Berkeley campus. In addition, general demand at UC Berkeley for a fast and sensitive confocal imaging system is likely to be very high because currently available conventional laser scanning microscopes are very heavily used, which is slowing the progress of NIH- funded research. To ensure the successful operation and support of the instrument and to broaden the potential user base when the instrument is not occupied by the major users, the microscope will be incorporated into the Molecular Imaging Center (MIC), an existing microscopy facility that is jointly supported by the Department of Molecular and Cell Biology, the Cancer Research Laboratory, and the Helen Wills Neuroscience Institute. The MIC will oversee training, operation and maintenance for the instrument. This plan is will enable the instrument to support the NIH-funded research of the major users while at the same time maximizing the potential to train and support new users on the UC Berkeley campus.

描述（由申请人提供）：来自加利福尼亚大学伯克利分校的六个NIH资助的细胞和开发生物学家，以及UC Berkeley分子成像中心（MIC），请求资金购买旋转磁盘共核显微镜系统，用于实时和时间隔离的动态细胞和发育过程。旋转磁盘共聚焦显微镜已成为成像动态细胞和发育过程的独特而有力的工具，包括细胞骨架动力学，膜运输，核转运，病原体感染和组织形态发生。这些仪器采用平行的多光束扫描方法，与标准的激光扫描共聚焦显微镜相比，可以显着更快地采集图像，从而实现了在非常快速的时间尺度上发生的细胞过程的成像。此外，当与非常敏感的电子相结合时，此类系统具有在低照明水平上获取图像的附加优势，从而最大程度地减少了对样品的光损伤，并允许扩展活细胞和组织的成像。尽管对旋转磁盘显微镜的访问对于该应用程序中的六个主要用户以实现其NIH资助的研究的目标至关重要，但是目前尚无此类系统可用于在UC Berkeley校园内使用。此外，加州大学伯克利分校对快速和敏感的共聚焦成像系统的一般需求可能非常高，因为当前可用的常规激光扫描显微镜非常大量使用，这正在减缓NIH资助的研究的进度。为了确保仪器的成功运行和支持并在主要用户未占用仪器时扩大潜在用户群，将将显微镜纳入分子成像中心（MIC），这是一个由分子和细胞生物学系共同支持的现有显微镜设施，癌症研究实验室，癌症研究实验室以及Helen Neuroscience Institute。麦克风将监督仪器的培训，操作和维护。该计划将使该工具能够支持主要用户的NIH资助研究，同时最大程度地提高培训和支持加州大学伯克利分校校园的新用户的潜力。