JEOL JEM-1400 Transmission Electron Microscope

JEOL JEM-1400 透射电子显微镜

• 批准号: 7780652
• 负责人: MICHAEL P KOONCE
• 金额: $ 50万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-27 至 2011-05-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7780652
• 关键词: 14 year old; Commit; Contract Services; Electron Microscope; Electron Microscopy; Ensure; Equipment; Funding; Laboratories; Life; Maintenance; Microscope; Microtomy; Pump; Records; Research; Research Personnel; Scientist; Services; Specimen; Staging; Time; Work; cost; digital; experience; functional improvement; instrument; operation; particle; tool; transmission process

DESCRIPTION (provided by applicant): A group of investigators at the Wadsworth Center are requesting funds to purchase a JEOL JEM-1400 transmission electron microscope (TEM). The major users of this instrument include five NIH-funded laboratories with extensive track records in, and whose specific aims require analyses by electron microscopy using multiple conventional thin-section and cryo approaches. Until the end of 2008, two microscopes at Wadsworth were available for these projects. One of these, a 24-year-old Philips EM420, has reached the end of its service life, and was decommissioned at the end of 2008. The other TEM, a 14- year-old Zeiss 910 that, despite being covered by a comprehensive service plan, has become so unreliable that its downtime (66% in 2008) represents a significant barrier to research progress. Moreover, the Zeiss 910 cannot be used for the cryo work formerly performed on the EM420. The JEOL JEM-1400 is an ideal replacement for the Zeiss 910, because it will readily accommodate both conventional and cryo modes of operation. This instrument is vital as it accepts a specimen holder that can load up to five grids at one time. For the three major users that perform extensive serial-section analyses, this feature represents a significant functional improvement. A cost-effective 2k x 2k digital camera is also included, particularly for the tomographic and single-particle cryo-EM projects. This instrument will be integrated into a long- standing EM Core, with local administrative and oversight committees to ensure appropriate use and maintenance. The Wadsworth Center is strongly committed to the addition of the JEM-1400. As evidence of support, the Center will provide a State-funded, experienced, Research Scientist to oversee the instrument's operation, assume costs associated with the annual service contract, and provide additional funds to purchase the cryo stage, controller, and dry pumping station equipment that are required for three of the five major users. In addition, and as time permits, this instrument will be made available to other funded investigators with assistance from a dedicated EM Core, thus enhancing the Wadsworth Center's long-standing use of electron microscopy as a research tool.

描述（由申请人提供）：Wadsworth中心的一组调查员要求资金购买JEOL JEOL JEM-1400传输电子显微镜（TEM）。该乐器的主要用户包括五个具有广泛记录的NIH资助的实验室，其具体目的需要通过电子显微镜进行多个常规薄片和冷冻方法进行分析。直到2008年底，沃兹沃思（Wadsworth）的两个显微镜可用于这些项目。其中之一是24岁的飞利浦EM420，已经达到了其服务寿命的终结，并于2008年底退役。另一个TEM是14岁的Zeiss 910，尽管受到了全面的服务计划的涵盖，但已变得如此不可靠，以至于其停机时间（2008年66％）代表了研究进度的重大障碍。此外，Zeiss 910不能用于以前在EM420上进行的冷冻工作。 JEOL JEM-1400是Zeiss 910的理想替代品，因为它很容易适应常规和冷冻操作模式。该仪器至关重要，因为它接受一个标本持有人，该标本持有人一次可以一次加载五个网格。对于执行广泛串行分析的三个主要用户，此功能代表了一个显着的功能改进。还包括一个具有成本效益的2K x 2K数码相机，尤其是用于断层扫描和单粒子冷冻EM项目。该工具将与当地的行政和监督委员会结合在一起，以确保适当的使用和维护。沃兹沃思中心强烈致力于增加JEM-1400。作为支持的证据，该中心将提供由国家资助，经验丰富的研究科学家来监督乐器的运营，承担与年度服务合同相关的成本，并提供额外的资金来购买五个主要用户中三个所需的冷冻阶段，控制器和干泵站设备。此外，随着时间的允许，该工具将在专用EM核心的帮助下向其他资助的研究人员提供，从而增强了Wadsworth中心长期使用电子显微镜作为研究工具。