MicroCT Core Facility in the Forsyth Institute

福赛斯研究所的 MicroCT 核心设施

• 批准号: 7795457
• 负责人: HAJIME SASAKI
• 金额: $ 29.46万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795457
• 关键词: Address; Area; Award; Bone Tissue; Boston; Collaborations; Commit; Core Facility; Data; Dental Research; Development; Employee; Funding; Future; Goals; Grant; Housing; Institutes; Leadership; Measures; Methods; Mutant Strains Mice; Oral; Participant; Principal Investigator; Reliance; Research; Research Personnel; Research Training; Sampling; Science; Scientist; Strategic Planning; Structure; System; Technology; Tissues; Tooth structure; United States National Institutes of Health; Universities; X-Ray Computed Tomography; bone; cost; craniofacial; experience; improved; instrument; meetings; programs

DESCRIPTION (provided by applicant): The Forsyth Institute affiliated with Harvard University is the world's preeminent organization dedicated to research and training in the areas of oral and craniofacial science. Teeth and the bony structures to which they are connected are central to most aspects of dental research. The three-dimensional x-ray data generated by microCT and the associated analyses provide the most quantitative, accurate, and efficient method to measure pathological and developmental changes in bones and teeth. The lack of easy access to microCt analyses has been an impediment to many researchers at Forsyth, resulting in reliance on long- distance collaborations or alternative methods to quantify these hard tissue changes. Thus, there is a strong need for convenient and inexpensive access to microCT analysis of bone and tooth samples by Forsyth researchers. The goal of this proposal is to meet this need by acquiring in house ex-vivo microCT capability. 66% of the PIs at Forsyth plan to use this instrument, attesting to the importance of mineralized tissue analyses at Forsyth. The Scanco 50 micro computed tomography (Scanco 50 microCT) instrument will enable more precise and comprehensive observations of the many significant projects that are ongoing at Forsyth (i.e. improved analysis of mutant mouse bone tissue) and provide investigators at Forsyth with the opportunity to easily and cost-effectively use this important technology in their research programs. Other microCT systems in the Boston area are either unavailable to our investigators or have reached maximum capacity. A total of 18 investigators are participating in this project. 12 investigators with 14 active NIH- funded Projects and current year direct grant awards will benefit from this proposed microCT shared Instrument grant (SIG). The participants in this application represent 62% of the NIH-funded projects in the Institute. Other Forsyth employees and neighboring scientists will also benefit. The proposed ex vivo microCT instrument will greatly expand and improve existing and pending NIH-supported projects, and it will address future needs created by the planned expansion of our research programs as dictated by our Strategic Plan. The Principal Investigator and Co-PIs have extensive experience with microCT scanners and will provide leadership and assistance for instrument's proper use. A Research Oversight Committee will provide overall direction and guidance. The Forsyth Institute is fully committed to support the proposal.

描述（由申请人提供）：哈佛大学隶属于福赛斯研究所，是世界上杰出的组织，致力于口头和颅面科学领域的研究和培训。牙齿及其连接的骨结构对于牙科研究的大多数方面都是核心。由MicroCT生成的三维X射线数据和相关的分析提供了最定量，准确，有效的方法，用于衡量骨骼和牙齿中的病理和发育变化。缺乏轻松地访问微观分析的是福赛斯的许多研究人员的障碍，从而依赖长距离协作或替代这些硬组织变化的替代方法。因此，福赛斯研究人员非常需要方便且廉价地访问骨骼和牙齿样品的微观分析。该提案的目的是通过在众议院前体内微分光度能力中获得这一需求。福赛斯（Forsyth）有66％的PI计划使用该仪器，证明了福赛斯（Forsyth）的矿化组织分析的重要性。 Scanco 50微型计算机断层扫描（Scanco 50 Microct）仪器将使福赛斯正在进行的许多重要项目（即改进突变小鼠骨组织的分析）更加精确，全面地观察，并为Forsyth的调查人员提供机会，并在其研究计划中轻松地使用这项重要技术。波士顿地区的其他Microct系统要么无法获得我们的研究人员，要么达到了最大容量。共有18名调查人员参加了该项目。 12个具有14个活跃NIH资助项目和当年直接赠款奖项的调查人员将受益于该拟议的Microct共享工具赠款（SIG）。该申请中的参与者占研究所NIH资助的62％的参与者。其他福赛斯员工和邻近科学家也将受益。拟议的离体Microct工具将大大扩展和改善现有的和未决的NIH支持项目，它将满足我们的战略计划所规定的计划扩展我们的研究计划所产生的未来需求。首席调查员和Co-PI在Microct扫描仪上具有丰富的经验，并将为仪器的适当使用提供领导和帮助。研究监督委员会将提供整体指导和指导。福赛斯研究所（Forsyth Institute）完全致力于支持该提案。

项目成果

Immunomodulation Mediated by Azithromycin in Experimental Periapical Inflammation.

• DOI: 10.1016/j.joen.2020.07.028
• 发表时间: 2020-11
• 期刊: Journal of endodontics
• 影响因子: 4.2
• 作者: Andrada AC;Azuma MM;Furusho H;Hirai K;Xu S;White RR;Sasaki H
• 通讯作者: Sasaki H

Activation of hypoxia-inducible factor 1 attenuates periapical inflammation and bone loss.

• DOI: 10.1038/s41368-018-0015-0
• 发表时间: 2018-04-13
• 期刊: International journal of oral science
• 影响因子: 14.9
• 作者: Hirai K;Furusho H;Hirota K;Sasaki H
• 通讯作者: Sasaki H

Elevated CD14 (Cluster of Differentiation 14) and Toll-Like Receptor (TLR) 4 Signaling Deteriorate Periapical Inflammation in TLR2 Deficient Mice.

• DOI: 10.1002/ar.23383
• 发表时间: 2016-09
• 期刊: Anatomical record (Hoboken, N.J. : 2007)
• 作者: Rider D;Furusho H;Xu S;Trachtenberg AJ;Kuo WP;Hirai K;Susa M;Bahammam L;Stashenko P;Fujimura A;Sasaki H
• 通讯作者: Sasaki H