Printing patterned substrates for analysis of axonal growth and regeneration:app

打印图案化基底用于分析轴突生长和再生：app

• 批准号: 7793044
• 负责人: Itzhak Fischer
• 金额: $ 9.2万
• 依托单位: DREXEL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793044
• 关键词: Address; Adult; Axon; Biocompatible Materials; Biological Models; Biomedical Engineering; Cell Differentiation process; Cells; Complex; Deposition; Development; Environment; Growth Cones; Hydrogels; In Vitro; Injury; Lead; Molecular; Natural regeneration; Neurodegenerative Disorders; Neuronal Differentiation; Neurons; Neurosciences; Pattern; Physiological; Printing; Research Personnel; Science; Signal Transduction; Signaling Protein; Site; Stem cells; Surface; Synapses; Therapeutic; axon growth; cell motility; design; improved; in vivo; injured; nano; nanoscale; neuromuscular; research study; response; scaffold; single molecule; stem cell biology; synaptogenesis; treatment strategy

DESCRIPTION (provided by applicant): The BioForce Nano eNabler 4.6 is a molecular printer that can be used to produce patterned molecular substrates on a variety of surfaces, including 3D surfaces. The nanoscale precision and versatility to deposit practically any biomaterial onto a large variety of surfaces is makes the Nano eNabler unique. In the experiments proposed here, the Nano eNabler will be used to address questions regarding the response of neurons to signaling proteins encountered during development and regeneration. The questions will be approached from different angles as a diverse group of researchers from the fields of cellular neuroscience, stem cell biology with focus on neurodegenerative disorders, and bioengineering/material science will investigate mechanisms of cell differentiation and process and synapse formation in vitro. Ultimately, the results obtained in these studies will be used to design in vivo treatment strategies that lead to improved regeneration in the adult injured or diseased CNS. Experiments of all users will involve the printing of single molecules and complex multicomponent patterns of molecules onto physiological or bioengineered substrates to investigate underlying signaling mechanisms implicated in (1) neuron differentiation and neuronal subtype specification, (2) axon dynamics during development (growth cone motility, axon extension, guidance and branching), (3) dendritic arbor formation, (4) synapse formation and plasticity (using the neuromuscular synapse as a model system), and (5) stem cell/neuron responses to inhibitory molecule combination resembling the adult injured CNS environment. In addition, the Nano eNabler will be used to screen components for incorporation in 3D hydrogel scaffolds that will serve as multifunctional platforms allowing the delivery of cells and therapeutic factors to the injury site. )

描述（由申请人提供）：生物弗洛斯纳米启用器4.6是一种分子打印机，可用于在包括3D表面在内的各种表面上产生图案化的分子底物。将任何生物材料沉积到各种表面上的纳米级精度和多功能性使纳米启用器变得独一无二。在此处提出的实验中，纳米支持器将用于解决有关神经元对发育和再生过程中遇到的信号蛋白反应的问题。作为来自细胞神经科学领域的多元化研究人员，重点关注神经退行性疾病的干细胞生物学，生物工程/材料科学将研究细胞分化的机制和过程的机制，并在体外，将研究从不同角度来解决这些问题。最终，在这些研究中获得的结果将用于设计体内治疗策略，从而改善了成人受伤或患病的中枢神经系统的再生。 Experiments of all users will involve the printing of single molecules and complex multicomponent patterns of molecules onto physiological or bioengineered substrates to investigate underlying signaling mechanisms implicated in (1) neuron differentiation and neuronal subtype specification, (2) axon dynamics during development (growth cone motility, axon extension, guidance and branching), (3) dendritic arbor formation, （4）突触形成和可塑性（使用神经肌肉突触作为模型系统），（5）干细胞/神经元对抑制性分子组合的反应类似于成人受伤的CNS环境。此外，纳米启用器将用于筛选成分，以将其掺入3D水凝胶支架中，该型水凝胶支架将用作多功能平台，允许将细胞和治疗因子输送到损伤部位。 ）