Typhoon Imaging System for Molecular Nutrition Research

用于分子营养研究的台风成像系统

• 批准号: 7791561
• 负责人: David J Eide
• 金额: $ 12.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-27 至 2011-09-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791561
• 关键词: Area; Arts; Aspergillus Nuclease S1; Autoradiography; Biochemistry; Biological Markers; Development; Diabetes Mellitus; Disease; EMSA; Ethanol Metabolism; Faculty; Film; Funding; Funding Applicant; Gastrointestinal Physiology; Genetic; Grant; Health; Healthcare; Homeostasis; Human; Image; Immunoblotting; Inferior; Investigation; Label; Laboratory Organism; Leadership; Medicine; Metabolic; Metabolism; Methods; Minerals; Molecular; Molecular Biology; Molecular Biology Techniques; Neonatal; Northern Blotting; Nuclease Protection Assays; Nutrient; Nutritional Study; Obesity; Physiologic pulse; Physiology; Protein Analysis; Proteins; RNA analysis; Regulation; Research; Research Personnel; Resources; Ribonucleases; Sampling; Scanning; Scientist; Staining method; Stains; System; Training; Training Programs; Two-Dimensional Polyacrylamide Gel Electrophoresis; United States National Institutes of Health; Universities; Wisconsin; base; insight; instrument; instrumentation; lipid metabolism; nutrition; prenatal; programs; public health relevance; research study; tool

DESCRIPTION (provided by applicant): Funds are requested to purchase a Typhoon 9400 Imaging System from GE Healthcare Biosciences Corporation for integration into the Interdepartmental Graduate Program in Nutritional Sciences (IGPNS) shared instrumentation resource. The IGPNS at the University of Wisconsin-Madison encompasses more than 40 faculty trainers from 16 different departments (Nutritional Sciences, Biochemistry, Genetics, Physiology, Medicine, etc.). The IGPNS is recognized as one of the top nutritional sciences training programs worldwide and is a premier program in the area of molecular nutritional sciences. This program is supported, and has been for many years, by an NIH T32 (2T32-DK007665-16) training grant. To maintain and advance our leadership in this field and to continue to train the most promising young scientists, it is essential that we have access to the state-of-the-art instrumentation and facilities on which our research depends. Imaging systems like the Typhoon 9400 have become essential research tools in molecular biology regardless of the particular experimental organism or particular problem under investigation. This instrument can be used to achieve extremely sensitive and quantitative analysis of samples generated by a wide variety of molecular biology techniques including DNA/RNA analysis (EMSA, Northern blots, RNase and S1 nuclease protection assays, etc) and protein analysis (immunoblotting, 35S metabolic labeling and pulse-chase experiments, protein staining following 1D and 2D PAGE, etc). Previously, we have largely relied on other methods (e.g., film-based autoradiography) that are far inferior to imager scanning and much more costly. The Typhoon 9400 Imager will fill this great need. Seven major users are submitting this proposal on behalf of the entire IGPNS. Six of these seven have current NIH funding. Public Health Relevance: The ongoing studies of the major users are yielding important insights into molecular aspects of nutrition as they relate to human health and disease. These include issues of mineral metabolism and homeostasis, biomarkers of nutrient status, alcohol metabolism and its effects on prenatal and neonatal development, gastrointestinal physiology and function, and the regulation of lipid metabolism and its relationship to obesity and diabetes. We will also attract new users from within the IGPNS; so an accessible imager will broaden and strengthen the research programs of researchers beyond the listed applicants. All IGPNS faculty are engaged in research of great significance to human health and therefore of importance to the aims of NIH.

描述（由申请人提供）：要求资金从GE Healthcare Biosciences Corporation购买台风9400成像系统，以将营养科学部门间研究生计划（IGPNS）集成为共享仪器资源。威斯康星大学麦迪逊分校的IGPN包括来自16个不同部门的40多位教师教练（营养科学，生物化学，遗传学，生理学，医学等）。 IGPN被认为是全球最高的营养科学培训计划之一，并且是分子营养科学领域的主要计划。该计划得到了NIH T32（2T32-DK007665-16）培训赠款的支持，并且已经支持了很多年。为了维持和提高我们在这一领域的领导地位，并继续培训最有前途的年轻科学家，至关重要的是，我们必须获得我们的研究所依赖的最先进的仪器和设施。诸如Typhoon 9400之类的成像系统已成为分子生物学的重要研究工具，无论其特定的实验生物或正在研究的特定问题如何。该仪器可用于实现由多种分子生物学技术产生的样品（包括DNA/RNA分析）（EMSA，北印迹，RNase和S1核酸酶保护分析）和蛋白质分析（35s抗体，35S代谢标记和脉冲结构实验，蛋白质的实验，蛋白质及2 page page and Page and Page）。以前，我们在很大程度上依赖其他方法（例如，基于膜的放射自显影），这些方法远不如成像器扫描，并且成本更高。台风9400成像仪将满足这一巨大需求。七个主要用户代表整个IGPN提交了该建议。这七个中有六个具有当前的NIH资金。公共卫生相关性：对主要用户的持续研究正在对与人类健康和疾病有关的营养分子方面产生重要的见解。其中包括矿物质代谢和稳态的问题，营养状况的生物标志物，酒精代谢及其对产前和新生儿发育的影响，胃肠道生理和功能以及脂质代谢的调节及其与肥胖和肥胖和糖尿病的关系。我们还将吸引来自IGPN内部的新用户；因此，可访问的成像师将扩大和加强所列申请人以外的研究人员的研究计划。所有IGPN的教师都从事对人类健康具有重要意义的研究，因此对NIH的目标至关重要。