Epigenetic Regulation of Adipogenesis

脂肪生成的表观遗传调控

• 批准号: 7967813
• 负责人: Kai Ge
• 金额: $ 26.23万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7967813
• 关键词: Accounting; Acetylation; Biological Assay; Diabetes Mellitus; Disease; Enzymes; Epigenetic Process; Functional RNA; Gene Activation; Gene Expression; Gene Expression Regulation; Histone Acetylation; Histone H3; Histones; Lipodystrophy; Lysine; Methylation; Methyltransferase; Morbidity - disease rate; Non-Insulin-Dependent Diabetes Mellitus; Obesity; PPAR gamma; Phase; Play; Regulation; Repression; Risk Factors; Role; Site; chromatin remodeling; histone modification; lipid biosynthesis; mortality; promoter

Specific Aim 1: examine the change of histone modifications on PPARgamma promoter in the early phase of adipogenesis. By ChIP assays, we have observed robust change of multiple histone acetylation and methylation on PPARgamma promoter in the early phase of adipogenesis. Specific Aim 2: investigate the functional significance of the histone modification change on PPARgamma promoter by depleting the responsible enzymes. Specific Aim 3: investigate the roles of other epigenetic mechanisms, in particular chromatin remodeling and non-coding RNAs, in regulation of PPARgamma expression and adipogenesis.

具体目标1：检查在脂肪生成的早期阶段，对PPARGAMMA启动子的组蛋白修饰的变化。通过CHIP分析，我们已经观察到在脂肪生成的早期阶段，多种组蛋白乙酰化和甲基化对ppargamma启动子的稳健变化。 特定目标2：研究组蛋白修饰变化对PPARGAMMA启动子的功能意义，通过耗尽负责的酶。 具体目标3：研究其他表观遗传机制的作用，特别是染色质重塑和非编码RNA，在调节ppargamma表达和脂肪形成中的作用。