Study the role(s) of p53 in embryonic stem cells

研究 p53 在胚胎干细胞中的作用

• 批准号: 7966127
• 负责人: Jing Huang
• 金额: $ 101.54万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7966127
• 关键词: Biological Models; DNA Damage; Development; Embryo; Future; Gene Chips; Generations; Genome; Genome Stability; Goals; Inner Cell Mass; Investigation; Malignant Neoplasms; Organism; Pathway interactions; Play; Process; Role; Signal Pathway; Stress; TP53 gene; Testing; Tissues; blastocyst; cell type; coping; embryonic stem cell; in vivo; mouse model; novel; stem; tumorigenesis

During fiscal year of 2009, we used ChIP-chip and gene expression microarray approaches to examine the role of p53 in embryonic stem cells. We surprisingly found that p53 regulates the Wnt sginaling pathway in embryonic stem cells. The Wnt signaling pathway plays important role in development and tumorigenesis. The underlying mechanism is currently under investigation in the lab. In the future, we plan to use in vivo mouse model to test our hypothesis that p53 has dual functions in embryonic stem cells.

在 2009 财年，我们使用 ChIP 芯片和基因表达微阵列方法来检查 p53 在胚胎干细胞中的作用。我们惊讶地发现 p53 调节胚胎干细胞中的 Wnt 信号通路。 Wnt信号通路在发育和肿瘤发生中发挥重要作用。目前实验室正在研究其潜在机制。未来，我们计划使用体内小鼠模型来验证我们的假设，即p53在胚胎干细胞中具有双重功能。