IMMUNE MONITORING LAB

免疫监测实验室

基本信息

批准号：
7944763
负责人：
LELAND H HARTWELL
金额：
$ 23.75万
依托单位：
FRED HUTCHINSON CANCER RESEARCH CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-03-10 至 2013-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944763
关键词：
Adoptive Transfer Affinity Alleles Analysis of Variance Antibodies Antigens B-Lymphocytes Binding Biological Biological Assay Biological Testing Cancer Center Support Grant Cancer Patient Cell Separation Cell physiology Cell surface Cells Cellular Assay Chromium Circular DNA Clonal Expansion Complex Consultations Cytolysis Cytotoxic T-Lymphocytes DNA DNA Sequence Rearrangement Data Analyses Dendritic Cells Development Enzyme-Linked Immunosorbent Assay Evaluation Excision Experimental Designs Flow Cytometry Fluorescent Dyes Fluorochrome Frequencies Gel Chromatography Genetic Polymorphism Genome Gerda brand of difluprednate HLA-A gene HLA-B Antigens HLA-C Antigens Housing Human Human Resources Immune Immune response Immunologic Monitoring Immunologics Immunology Immunotherapy In Vitro Individual Knowledge Label Laboratories Length Lymphocyte MHC Class I Genes Macaca Malignant Neoplasms Measurement Measures Mediator of activation protein Methodology Methods Microspheres Mitogens Mixed Lymphocyte Culture Test Molecular Molecular Analysis Mus Names Natural Killer Cells Nature Output Peptide/MHC Complex Peptides Performance Peripheral Peripheral Blood Mononuclear Cell Phenotype Population Procedures Production Proliferating Proteins Radioimmunoassay Reaction Reader Reagent Relative (related person)Request for Applications Research Research Personnel Resource Sharing Resources Reverse Transcriptase Polymerase Chain Reaction Role Sampling Services Spottings Staining method Stains Stem cell transplant Streptavidin Structure T-Cell Receptor T-Cell Receptor-Rearrangement Excision DNA Circles T-Lymphocyte TRB@ gene cluster Techniques Technology Thymidine Thymus Gland Time Training Universities Washington antibody conjugate base beta Chain Antigen T Cell Receptor beta-2 Microglobulin cell mediated lymphocytolysis test complementarity-determining region 3 cytokine design enzyme linked immunospot assay killings monocyte monomer reconstitution response tool

项目摘要

The Immune Monitoring Lab provides investigators with a broad spectrum of services and expertise to carry out a range of assays of human proteins and other biological mediators and perform studies where precise evaluations of the immunologic response are required. Resource services include the production of peptide- MHC Class-l tetramers, unique reagents for accessing antigen-specific T cells; molecular assays for T cell receptor (TCR) related analysis, cellular assays for determining cellular function and analytical services using ELISA and Luminex microbead methodology. This application requests continued support for a resource which fulfills an essential role for research within the Fred Hutchinson/University of Washington Cancer Consortium.

免疫监测实验室为研究人员提供广泛的服务和专业知识对人类蛋白质和其他生物介质进行一系列测定，并在精确的情况下进行研究需要评估免疫反应。资源服务包括肽的生产 MHC I 类四聚体，用于接触抗原特异性 T 细胞的独特试剂； T 细胞分子检测受体 (TCR) 相关分析、用于确定细胞功能的细胞测定和分析服务使用 ELISA 和 Luminex 微珠方法。此应用程序请求继续支持在 Fred Hutchinson/华盛顿大学的研究中发挥重要作用的资源癌症联盟。