Molecular Screening Shared Resouce

分子筛选共享资源

• 批准号: 7944609
• 负责人: Kenneth Alan Bradley
• 金额: $ 14.96万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-02 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944609
• 关键词: Animal Model; Area; Biochemical; Biological; Biological Assay; Budgets; California; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cellular biology; Characteristics; Community Clinical Oncology Program; Consultations; Development; Equipment; Funding; Gene Expression Regulation; Genitourinary system; Hematopoietic Neoplasms; Human; Incubators; Individual; Institutes; Instruction; Jonsson Comprehensive Cancer Center of University of California Los Angeles; Knock-out; Leadership; Letters; Libraries; Molecular; Molecular Epidemiology; Mus; Proteins; RNA Sequences; RNA library; Reader; Recording of previous events; Reporting; Resource Sharing; Resources; Robotics; Running; Science; Screening procedure; Signal Transduction; Technology; Therapeutic; Thoracic Oncology; United States National Institutes of Health; Validation; Yeasts; Zebrafish; cancer cell; carcinogenesis; college; follow-up; genome sequencing; genome wide association study; genome-wide; high throughput screening; medical schools; meetings; member; nanosystems; programs; small molecule; small molecule libraries; tumor immunology

The Molecular Screening Shared Resource (MSSR): Completion of the genome sequences of humans, mice, zebrafish and a number of other model organisms has led to the tentative identification of all encoded proteins in these species. The proliferation both of chemical libraries and of libraries of genome wide inhibitory RNA sequences for a number of species has made it possible to conceive of looking for inhibitory small molecules and inhibitory RNAs for all genetically encoded targets. The concomitant development of High Throughput Screening (NTS) technologies has made it possible - with the right resources - to move this from concept to practice. The UCLA JCCC Molecular Screening Shared Resource (MSSR) was established in 2003 to meet this exciting scientific objective. The MSSR now has dedicated space in the new California NanoSystems Institute, small molecule libraries containing over 70,000 compounds, genomewide knockout libraries for yeast, and interfering RNA libraries for both murine and human targets. Excellent academic and technical leadership has established a robust, accessible screening center that assists both in developing reliable and robust enzymatic and cell culture HTS assays and in facilitating genome-wide screens. The David Geffen School of Medicine, the California NanoSystems Institute (CNSI), the UCLA College of Letters and Science, several individual departments and some private donors have joined the JCCC to provide funding for the equipment and libraries (well over $1,000,000) and operating "start-up" required to get the MSSR up and running. The Shared Resource has run (or is currently running) 65 screens, the great majority of which are for JCCC members. Members of the Gene Regulation, Signal Transduction and Therapeutics, Tumor Immunology, Cancer Cell Biology, Hematopoietic Malignancies, Developing Program in Molecular Epidemiology and Carcinogenesis, Thoracic Oncology and Genitourinary Oncology Program Areas have already utilized this Shared Resource. The CCSG budget request for this Shared Resource for the first year CCSG budget is for 13% of the estimated operating budget; support for the shared resource also comes from the CNSI, an NIH Center for Radioprotectors, the UCLA Chancellor's Biosciences Initiative, user recharges and philanthropy. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). Eight Cancer Center members representing four Cancer Center Program Areas completed screens in the Molecular Screening Shared Resource during the reporting period; many screens are ongoing. This is a continuing shared resource.

分子筛选共享资源（MSSR）：人类基因组序列的完成， 小鼠，斑马鱼和许多其他模型生物已导致所有编码的暂定识别 这些物种中的蛋白质。化学文库和基因组宽的文库的扩散 许多物种的抑制性RNA序列使人们有可能寻找抑制性 用于所有遗传编码靶标的小分子和抑制性RNA。伴随的发展 高吞吐量筛查（NTS）技术使得（有了正确的资源）可以移动 从概念到实践。 UCLA JCCC分子筛选共享资源（MSSR）为 成立于2003年，旨在满足这个令人兴奋的科学目标。 MSSR现在在 新加利福尼亚纳米系统研究所，小分子库，其中包含70,000多种化合物，全基因组 酵母的敲除文库，并干扰鼠类和人类靶标的RNA库。出色的 学术和技术领导力已经建立了一个健壮，可访问的筛查中心，以协助 开发可靠且可靠的酶促和细胞培养HTS分析并促进全基因组促进 屏幕。加利福尼亚州纳米系统研究所（CNSI）的大卫·格芬医学院，加州大学洛杉矶分校 文学与科学学院，几个各个部门和一些私人捐助者加入了 JCCC为设备和图书馆提供资金（超过$ 1,000,000）并运营“启动” 需要使MSSR启动并运行。共享资源已运行（或当前正在运行）65 屏幕，其中大多数是JCCC成员。基因调控的成员，信号 转导和治疗学，肿瘤免疫学，癌细胞生物学，造血恶性肿瘤， 开发分子流行病学和癌变，胸腔肿瘤学和泌尿生殖器的程序 肿瘤计划领域已经利用了此共享资源。 CCSG预算要求 第一年的共享资源CCSG预算是估计运营预算的13％；支持 共享资源也来自CNSI，CNSI，NIH辐射保护区中心，UCLA总理的 生物科学计划，用户补给和慈善事业。 （另请参阅共享的第6.2.3节 历史上的资源，描述，基本特征）。 代表四个癌症中心计划区的八个癌症中心成员完成了屏幕 报告期间的分子筛选共享资源；许多屏幕正在进行中。 这是一个持续的共享资源。