Flow Cytometry

流式细胞仪

• 批准号: 7685024
• 负责人: Allan J Zajac
• 金额: $ 23.6万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7685024
• 关键词: Acquired Immunodeficiency Syndrome; Address; Alabama; Area; Arts; Basic Science; Biological Assay; Capital; Cell Separation; Cell physiology; Cells; Cellular Immunity; Cessation of life; Clinical; Clinical Trials; Collaborations; Communication; Communities; Consultations; Core Facility; Disease; Documentation; Education; Ensure; Equipment; Flow Cytometry; Fluorescence; Fostering; Free Will; Funding; Genetic Recombination; Goals; Grant; HIV; HIV-1; Hour; Image; Immune system; Individual; Infection; Laboratories; Laboratory Personnel; Methodology; Microscopy; Mission; Monitor; Needs Assessment; Nucleic Acids; Peptides; Phenotype; Population; Process; Property; Proteins; Publications; Reporter; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Resources; Running; Sampling; Services; Sorting - Cell Movement; Speed; Spottings; System; Systems Analysis; Technology; Time; Training; Training Activity; Training and Education; United States National Institutes of Health; Universities; Vaccine Design; Vaccines; Viral; Viral Pathogenesis; Virus Replication; Work; base; cost; cytokine; empowered; experience; flexibility; fluorescence microscope; improved; innovation; instrumentation; interest; investigator training; member; new technology; novel strategies; particle; programs; response; synthetic polymer Bioplex; tool; treatment strategy

The major objective of the University of Alabama (DAB) Center for AIDS Research (CFAR) Flow Cytometry Core is to provide state-of-the-art flow cytometry services to support AIDS research programs. Flow cytometry is a unique and powerful technology for analyzing the fluorescent properties of particles and can be readily applied to determining the phenotype and function of cells, as well as utilized for isolating defined cell populations by sorting. In recent years the use of flow cytometric analyses in HIV research has become ever more prominent as newer approaches for dissecting the interactions between HIV and cells of the immune system have been developed; fluorescent based reporter systems have been harnessed to monitor virus replication, reactivation, and recombination; and sophisticated technological advances in instrumentation have provided new platforms for high-speed polychromatic analyses and cell sorting. The CFAR flow cytometry core is, therefore, a vital and versatile resource for promoting and facilitating AIDS research. The flow cytometry core's mission is to maximize the benefits of this technology by providing the necessary instrumentation within a well organized, centralized, facility that is capable of handling potentially infectious material. It provides training opportunities and consultations, and it also serves as a conduit for fostering interactions between investigators and laboratory personnel with diverse interests and expertise. During the previous funding period the CFAR flow cytometry core has undergone a significant equipment upgrade, with the installation of a BDFACSAria cell sorter as well as a BD-LSR-II analytical flow cytometer. The flow cytometry core has also improved its visibility and accessibility as well as enhanced education and training initiatives. As a result of these endeavors the usage of the flow cytometry core has increased more than three-fold since 2002. The flow cytometry core has supported the research activities of 184 different individuals from 69 Principle Investigators laboratories and has contributed to basic, clinical, and translational initiatives in the areas of viral pathogenesis, anti-viral research, cellular immunity, and vaccine design. The core has supported the activities of 88 NIH grants and contributed to 172 publications The aims of the flow cytometry core are to now build upon our progress-to-date by further enhancing services, and education and training activities so that users are empowered to take full advantage of the sophisticated instrumentation that is available. These efforts include, based on a needs assessment analysis, the installation of a third flow cytometer and a BioPlex flow cytometer for multiplexed analysis of biomolecules (e.g. cytokines) in the beginning of 2008. We also aim to promote innovation by working with users, and fostering collaborations and crosscore partnerships that encourage cutting-edge AIDS related research activities.

阿拉巴马大学（DAB）艾滋病研究中心（CFAR）流式细胞仪核心的主要目标是 提供最先进的流式细胞仪服务以支持艾滋病研究计划。流式细胞仪是一种独特的， 强大的技术用于分析颗粒的荧光性能，并且可以轻易地应用于确定 细胞的表型和功能，以及用于通过分类来隔离定义的细胞群体。近年来 随着剖析的新方法 艾滋病毒与免疫系统细胞之间的相互作用已经开发出来。基于荧光的记者 已利用系统来监测病毒复制，重新激活和重组。和精致 仪器的技术进步为高速多色分析提供了新的平台和 细胞分类。因此，CFAR流式细胞仪核心是促进和促进的重要资源 艾滋病研究。流式细胞仪核心的使命是通过提供该技术的好处 井井有条，集中式设施中的必要仪器，该设施能够处理潜在的感染力 材料。它提供培训机会和咨询，也是促进互动的渠道 在研究人员和具有多种利益和专业知识的实验室人员之间。在以前的资金中 时期CFAR流式细胞仪核心进行了重要的设备升级，安装了BDFACSARIA 细胞分类器以及BD-LSR-II分析流式细胞仪。流式细胞术核心也改善了 可见性和可及性以及增强的教育和培训计划。由于这些努力 自2002年以来，流式细胞仪核的使用增加了三倍以上。流式细胞仪核有 支持来自69位主要研究人员实验室的184个不同人的研究活动，并已有 在病毒发病机理，抗病毒研究，细胞中有助于基本，临床和转化计划 免疫力和疫苗设计。核心支持了88个NIH赠款的活动，并为172做出了贡献 出版物流式细胞仪核心的目的现在是通过进一步增强来建立我们的进度 服务以及教育和培训活动，以便用户有权充分利用复杂的优势 可用的仪器。这些努力包括根据需求评估分析，安装 第三个流式细胞仪和生物分析的生物分析（例如细胞因子）的三流式流式细胞仪 2008年初。我们还旨在通过与用户合作并促进合作和交叉核心来促进创新 鼓励尖端艾滋病相关研究活动的伙伴关系。