Genome sequencing of lytic and temperate phages infecting members of the Roseobacter clade

感染玫瑰杆菌分支成员的裂解噬菌体和温带噬菌体的基因组测序

基本信息

批准号：
NE/F010044/1
负责人：
Hendrik Schaefer
金额：
$ 0.72万
依托单位：
University of Warwick
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2008
资助国家：
英国
起止时间：
2008 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=NE%2FF010044%2F1
关键词：
Genome sequencing lytic temperate phages

项目摘要

Marine bacteria are probably the most abundant group of organisms on the planet and are of pivotal importance in the major elemental cycles. Their abundance as biological entities is only exceeded by the viruses (phages) that infect them. Bacterially mediated transformations of organic and inorganic matter have important implications for atmospheric composition and climate regulation. The Roseobacter group is a clade of marine bacteria that is commonly found at high abundance throughout the oceans, and studies of a large number of cultivated representatives have shown this to be a physiologically diverse group. A methyl halide (methyl bromide and methyl chloride) and DMSP degrading strain, Roseovarius sp. 217, has recently been isolated at Warwick and the genome of this strain has been sequenced. Further genome sequences of strains from this clade are available now and allow to study these organisms in more detail at the proteomic and transcriptomics level. Marine viruses influence biogeochemical cycles through lysis of host cells, diverting carbon flow from heterotrophic protists into the pool of dissolved organic matter. Recently, phages infecting marine cyanobacteria have been shown to carry genes that encode proteins of the photosynthetic reaction centre of cyanobacteria. These genes were expressed during the phase of virus replication constituting virus-encoded photosynthesis in the marine environment. Phages infecting members of the ubiquitous Roseobacter clade (roseophages) may affect the extent of biogeochemical cycling carried out by Roseobacters, but these phage have received little attention until now. We have isolated three roseophages, including one that infects Roseovarius sp. 217, and propose to sequence the genomes of these three phages. This will inform us of particular features of these phage and will give us clues about their life strategy and evolution. It is not clear what these genome sequences will tell us, but given the exciting insights from cyanophage genomes, and considering the abundance, genetic and physiologic diversity of their hosts, the outcomes of this research will be very informative in the context of the evolution and ecology of Roseobacters and their phages and are very likely to give us exciting new insights into the properties of roseophages.

海洋细菌可能是地球上最丰富的生物群，在主要元素周期中至关重要。它们作为生物实体的丰富性仅被感染它们的病毒（噬菌体）超过。细菌介导的有机和无机物质转化对大气组成和气候调节具有重要意义。玫瑰杆菌是一组海洋细菌的进化枝，在整个海洋中通常很丰富，并且对大量耕种代表的研究表明这是一个生理上多样的群体。甲基卤化物（甲基溴和氯化甲基）和DMSP降解菌株，Roseovarius sp。 217最近在沃里克（Warwick）分离出来，该菌株的基因组已被测序。该进化枝的菌株的进一步基因组序列现已上市，可以在蛋白质组学和转录组水平上更详细地研究这些生物。海洋病毒通过释放宿主细胞的裂解影响生物地球化学循环，将碳流从异养生物转移到溶解有机物的池中。最近，已证明感染海洋蓝细菌的噬菌体可以携带编码蓝细菌光合反应中心的蛋白质的基因。这些基因在构成海洋环境中病毒编码的光合作用的病毒复制阶段表达。噬菌体感染无处不在的玫瑰杆菌（Roseophages）的噬菌体可能会影响玫瑰杆菌（Roseobacters）进行的生物地球化学循环的程度，但直到现在，这些噬菌体都没有受到关注。我们已经孤立了三个玫瑰噬细胞，其中包括一种感染Roseovarius sp。 217，并提出对这三个噬菌体的基因组进行测序。这将告知我们这些噬菌体的特定特征，并将为我们提供有关其生活策略和进化的线索。目前尚不清楚这些基因组序列会告诉我们什么，但是考虑到氰基基因组的令人兴奋的见解，并且考虑到宿主的丰度，遗传和生理多样性，这项研究的结果将非常有用，在Roseobacters and Phages的进化和生态中，这将是非常有用的，并且很可能使我们成为Rose的新洞察力。