INTRACELLULAR RNA TRAFFICKING

细胞内 RNA 运输

• 批准号: 7956387
• 负责人: JOHN H CARSON
• 金额: $ 8.14万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956387
• 关键词: Binding; Cell model; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Fluorescence; Funding; Grant; In Vitro; Institution; Life; Maps; Measures; Modeling; Neurons; Process; RNA; Research; Research Personnel; Resources; Source; Spectrum Analysis; Surface Plasmon Resonance; System; Techniques; United States National Institutes of Health; in vivo; trafficking; virtual

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. We have measured quantitative parameters for each molecular interaction in RNA trafficking using surface plasmon resonance analysis in vitro and fluorescence correlation spectroscopy (FCS) and in live cells. Quantitative parameters are used in Virtual Cell models of RNA trafficking.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 RNA运输是一个复杂的过程，特定的RNA位于特定的亚细胞隔室。通过各种体内和体外技术，我们描述了描述神经细胞中RNA运输系统的分子相互作用图。我们已经使用表面等离子体共振分析在体外和荧光相关光谱（FCS）和活细胞中测量了RNA运输中每个分子相互作用的定量参数。定量参数用于RNA运输的虚拟细胞模型。