STRUCTURAL ORIGINS OF NUCLEIC ACID SEQUENCE DISCRIMINATION BY PROTEINS

通过蛋白质区分核酸序列的结构起源

• 批准号: 7954344
• 负责人: JOHN J. PERONA
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954344
• 关键词: Base Sequence; Biological; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallization; Cysteine-tRNA ligase; DNA Restriction-Modification Enzymes; Discrimination; Enzymes; Funding; Gene Expression Regulation; Grant; Heart; Human; Hydrogenase; Institution; Isoenzymes; Methane; Nucleic Acids; Nucleic acid sequencing; Organism; Pathway interactions; Play; Production; Proteins; Research; Research Personnel; Resources; Role; Source; Structure; System; Transfer RNA; United States National Institutes of Health; mutant; novel; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability of proteins to discriminate among nucleic acid sequences is at the heart of gene regulation and translational control in all organisms. Here we propose detailed x-ray crystallographic studies of protein-DNA and protein-RNA complexes of bacterial, human, and archaeal origins which elucidate this common theme in a variety of important biological contexts. Systems to be studied for which crystal structures of components to a larger assembly have been determined, or for which native enzymes/mutants have been solved are the transcriptional regulator Lrp, the glutaminyl and cysteinyl-tRNA synthetases (CysRS), and the DNA restriction-modification enzymes M.HhaI and EcoRV. Newer projects for which crystals are available, or for which protein-nucleic acid complexes are being screened for crystallization, are the human CysRS, two enzymes from a novel tRNA biosynthetic pathway in M. mazei, and tRNA complexes with isozymes of a hydrogenase from the methanogenesis pathway of M. jannaschii, which may play a role in regulating anaerobic methane production.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 蛋白质在核酸序列中区分蛋白质的能力是所有生物体中基因调节和转化控制的核心。 在这里，我们提出了细菌，人和古细菌起源的蛋白质-DNA和蛋白-RNA复合物的详细X射线晶体学研究，这些蛋白-DNA和蛋白-RNA复合物在各种重要的生物学环境中阐明了这一共同主题。 要研究的系统已经确定了成分的晶体结构，或者已经解决了天然酶/突变体的晶体结构是转录调节剂LRP，谷氨酰胺基和胱烷基-TRNA合成酶（CYSRS），以及DNA限制性限制性化的enzymes M.hhai和Ecorv。 晶体可用的新项目，或为结晶筛选的蛋白质核酸络合物是人类CYSR，这是M. Mazei中新型TRNA生物合成途径的两个酶，以及与Jannaschii M. Jannaschii M. Jannaschi eybase的甲基化途径的同过程中的tRNA复合物，可能会扮演一位甲基甲基甲基甲基甲基甲烷的途径。