AXEL BRUNGER PRT TIME

阿克塞尔·布伦格 PRT 时间

• 批准号: 7954169
• 负责人: AXEL T BRUNGER
• 金额: $ 0.33万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954169
• 关键词: Collection; Complex; Computer Retrieval of Information on Scientific Projects Database; Dimensions; Dyes; Face; Funding; Gentian Violet; Goals; Grant; Institution; Measures; Property; Proteins; Research; Research Personnel; Resources; Source; Staining method; Stains; Time; Twin Multiple Birth; United States National Institutes of Health; research study; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed experiments involve the characterization of small protein crystals. The protein in question is a fairly large (~500 kDa) complex of two different proteins. The crystals stain blue in the presence of crystal violet dye, indicating that they are composed of protein. However, the crystals grow only as small plates, with dimensions of approximately 100-200 microns on the plate face and 50 microns measured orthogonal to the face. The crystals are to be studied to determine extent of diffraction, twinning and mosaicity. Potential improvements in cryo-conditions will also be examined. The crystals have been grown in a variety of different substrates. The effect of these substrates upon the diffraction properties will be studied as well. Determination of space group and the collection of a native set for these crystals would be the ultimate goal.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 拟议的实验涉及小蛋白质晶体的表征。 所讨论的蛋白质是两种不同蛋白质的相当大（约 500 kDa）的复合物。 晶体在结晶紫染料存在下呈蓝色，表明它们由蛋白质组成。 然而，晶体仅以小板的形式生长，板面上的尺寸约为 100-200 微米，垂直于该面测量的尺寸为 50 微米。将研究这些晶体以确定衍射、孪生和镶嵌性的程度。 还将研究低温条件的潜在改进。 这些晶体已在各种不同的基底上生长。 还将研究这些基底对衍射特性的影响。 确定空间群并收集这些晶体的原生集合将是最终目标。