Laser Activated Ophthalmic Adhesive

激光激活眼科粘合剂

基本信息

批准号：
7472807
负责人：
BARBARA Ann SOLTZ
金额：
$ 7.15万
依托单位：
CONVERSION ENERGY ENTERPRISES
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-07-01 至 2009-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7472807
关键词：
Adhesions Adhesives Anastomosis - action Animal Experiments Animal Model Animals Area Biocompatible Materials Biodegradation Blindness Blood Vessels Blunt Trauma Bulla Cataract Cataract Extraction Chemistry Clinical Clinical Trials Closure Collagen Conjunctival Pterygium Cornea Corneal Injury Corneal Ulcer Cross-Linking Reagents Cyanoacrylates Data Development Doctor of Philosophy Drug Formulations End Point Ensure Excision Extracapsular Eye Eye diseases Film Foundations Funding Glaucoma Glues Goals Grant Human Impairment In Situ In Vitro Institutes Investigation Keratoplasty Laboratory Research Laceration Lamellar Keratoplasty Laser In Situ Keratomileusis Lasers Lead Measures Mechanics Medical Electronics Membrane Methods Modeling National Eye Institute Object Attachment Operative Surgical Procedures Outcome Penetrating Keratoplasty Phase Phase I Clinical Trials Phase II Clinical Trials Postoperative Period Preparation Principal Investigator Procedures Process Property Proteins Psychological reinforcement Radial Keratotomy Rate Reaction Reagent Research Research Personnel Retinal Risk Rupture Sampling Secure Site Small Business Funding Mechanisms Small Business Innovation Research Grant Solutions Standards of Weights and Measures Sterility Sterilization for infection control Strabismus Surgical Flaps Surgical Models Surgical incisions Surgical sutures Survivors System Techniques Technology Temperature Tensile Strength Testing Thick Tissue Adhesives Tissues Trauma United States Dept. of Health and Human Services United States Food and Drug Administration United States National Institutes of Health Vision Welding Work anterior chamber articular cartilage base biomaterial compatibility cell injury commercialization conjunctiva corneal surgery design experience improved in vitro Model in vivo innovation pre-clinical pressure protein crosslink reconstruction repaired research study response seal sensor skills tissue glueing tissue soldering tissue welding wound

项目摘要

Project Summary The significance of this project is the development of a laser assisted tissue welding technique to close corneal wounds, provide a foundation for innovative treatment of eye disease and demonstrate the unique properties of a laser cured collagen adhesive to repair ophthalmic tissue with superior strength and stability. Millions of ophthalmic procedures are performed annually, most incorporating sutures to close and seal wounds. Yet this method can cause severe complications, can weaken the integrity of the eye and may pose a risk for ocular rupture in the case of blunt trauma. Other techniques to close wounds, including the use of tissue glues or grafting, can be even more problematic. Phase I studies were conducted to measure the biostability of solder formulations, evaluate tissue bonding strategies and compare wound stability as a function of intrachamber pressure in four in vitro models of clinical corneal surgery: radial keratotomy, LASIK flaps, penetrating keratoplasty and cataract excision. The results confirmed that the laser solder approach is superior to standard sutures with no evidence of tissue shrinkage. The strength of the soldered repair was dependent upon reaching a precise temperature set by choice of laser, solder composition and film thickness. Solder biostability is increased by reaction with a standard crosslinking reagent. The specific aim for Phase II is to optimize solder processing, laser techniques and surgical outcome in survivor animal surgical models. Task descriptions include preparation of sterile solder under stringent process controls, design and assembly of a compact preclinical laser systems, optimization of a laser handpiece and perform ex vivo and in vivo experiments on a lapine model. Surgical endpoints will include the quality of the wound seal, the extent, if any, of collateral thermal tissue damage, solder stability and biocompatibility.

项目概要该项目的意义在于开发激光辅助组织焊接技术来闭合角膜伤口，为眼部疾病的创新治疗奠定了基础，并展示了其独特的特性激光固化胶原蛋白粘合剂，用于修复眼部组织，具有卓越的强度和稳定性。数百万每年进行一次眼科手术，大多数采用缝合来闭合和密封伤口。然而这个该方法可能会导致严重的并发症，会削弱眼睛的完整性，并可能造成眼部疾病的风险钝器损伤时破裂。其他闭合伤口的技术，包括使用组织胶或嫁接的话，问题就更大了。进行第一阶段研究以测量焊料的生物稳定性配方，评估组织粘合策略并比较伤口稳定性与腔内函数的关系四种临床角膜手术体外模型中的压力：放射状角膜切开术、LASIK 皮瓣、穿透术角膜移植术和白内障切除术。结果证实，激光焊接方法优于标准缝合，没有组织收缩的证据。焊接修复的强度取决于通过选择激光、焊料成分和薄膜厚度达到精确设定的温度。焊接通过与标准交联剂反应可提高生物稳定性。第二阶段的具体目标是优化幸存者动物手术模型中的焊料加工、激光技术和手术结果。任务描述包括在严格的过程控制下制备无菌焊料、设计和组装紧凑的临床前激光系统、激光手机的优化以及离体和体内执行在兔子模型上进行的实验。手术终点将包括伤口密封的质量、程度（如果有）、附带的热组织损伤、焊料稳定性和生物相容性。