PHOSPHO-REGULATION OF THE YEAST NET1-CDC14 RENT COMPLEX

酵母 NET1-CDC14 租金复合物的磷酸调节

• 批准号: 7957704
• 负责人: CLARENCE S CHAN
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957704
• 关键词: Biology; Cell Cycle; Cell Nucleolus; Complex; Computer Retrieval of Information on Scientific Projects Database; Funding; Fungal Genome; Grant; Institution; Mass Spectrum Analysis; Phosphorylation; Phosphotransferases; Protein Kinase; Protein phosphatase; Proteins; Regulation; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Yeasts

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Net1 sequesters the Cdc14 protein phosphatase and other proteins in the yeast nucleolus through much of the cell cycle. The Cdc5 and Cdc28 protein kinases regulate release of Cdc14 from the Net1 complex. We would like to use mass spectrometry to identify other proteins that are released from the Net1 complex upon phosphorylation by additional kinases. This study should provide invaluable information on how multiple kinases regulate the sequestration of proteins in the nucleolus.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 NET1通过大部分细胞周期隔离酵母核仁中的Cdc14蛋白磷酸酶和其他蛋白质。 CDC5和CDC28蛋白激酶调节Cdc14从Net1复合物中释放。 我们想使用质谱法来鉴定其他激酶在磷酸化后从Net1复合物中释放出来的其他蛋白质。 这项研究应提供有关多种激酶如何调节核仁中蛋白质的隔离的宝贵信息。