THE EFFECT OF D-AMINO ACIDS ON THE FOLDING DYNAMICS OF TRP-CAGE

D-氨基酸对 TRP-CAGE 折叠动力学的影响

• 批准号: 7955457
• 负责人: Vikas Nanda
• 金额: $ 0.48万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955457
• 关键词: Amino Acids; Computer Retrieval of Information on Scientific Projects Database; Fluorescence; Funding; Goals; Grant; Individual; Institution; Kinetics; Laboratories; Nitriles; Optics; Process; Property; Proteins; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; absorption; design; protein folding; vibration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cyanophenylalanine (PheCN) has found many applications in infrared spectroscopic studies of protein folding dynamics due to the strong and spectrally isolated nitrile vibration. Recently we discovered that PheCN we recorded visible absorption and emission spectra and found good fluorescence property of this modified aminoacid. Typically, absorption is in the 270nm region and emission maximum around 310nm. We propose to use in protein folding studies as a local chromophor, especially in the study on the effect of D-amino acids on the folding dynamics of Trp-cage. D-amino acids are commonly used in protein design to enhance protein stabilities. However, the kinetic role of D-amino acids in protein folding has not been studied. Thus, the goal of this project is to use a small folding motif, Trp-cage, to systematically examine the effect of individual D-amino acids on the folding kinetics of the protein.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 由于腈振动强烈且光谱隔离，氰基苯丙氨酸 (PheCN) 在蛋白质折叠动力学的红外光谱研究中有许多应用。最近我们发现了PheCN，我们记录了可见吸收和发射光谱，并发现这种修饰的氨基酸具有良好的荧光性质。 通常，吸收位于 270 nm 区域，发射最大值在 310 nm 附近。我们建议在蛋白质折叠研究中用作局部发色团，特别是在 D-氨基酸对色氨酸笼折叠动力学影响的研究中。 D-氨基酸常用于蛋白质设计以增强蛋白质稳定性。然而，D-氨基酸在蛋白质折叠中的动力学作用尚未被研究。因此，该项目的目标是使用一个小的折叠基序 Trp-cage 来系统地检查单个 D-氨基酸对蛋白质折叠动力学的影响。