Do mixotrophic protists make oligotrophic oceanic gyres sustainable ecosystems?

混合营养原生生物能否使寡营养海洋环流成为可持续的生态系统？

• 批准号: NE/E016138/1
• 负责人: Mikhail Zubkov
• 金额: $ 39.13万
• 依托单位: NATIONAL OCEANOGRAPHY CENTRE
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FE016138%2F1
• 关键词: Do; mixotrophic; protists; make; oligotrophic

The aim of the proposal is to explain the functioning of the oligotrophic oceanic gyres, the Earth's largest ecosystems, which profoundly affect global biogeochemistry and climate. Compared to complex biogeochemical dynamics of C and N with their multiple inorganic pools that do not allow their complete microbial budgets to be accurately determined experimentally, a phosphate (P) budget in surface waters, particularly in the P depleted North Atlantic gyre, is easier to quantify because inorganic P has only one dissolved form in which it is taken up by microbes. The project proposes to experimentally test and to numerically model a new concept of a simplest ecosystem i.e. that: mixotrophic protists control the two dominant bacterioplankton populations, Prochlorococcus cyanobacteria and the SAR11 clade, competing for depleted inorganic P. Using a combination of outlined laboratory and oceanic cruise experiments the following hypotheses will be addressed (i) mixotrophic protists rather than heterotrophic protists dominate bacterial phagotrophy in oligotrophic waters; (ii) mixotrophic protists and not phototrophic protists or cyanobacteria dominate primary production in oligotrophic waters; (iii) protist phagotrophy rather than bacterioplankton senescent death / viral lysis dominate nutrient recycling in oligotrophic waters; (iv) an oligotrophic ecosystem controlled by mixotrophic protists is sustainable in terms of P recycling and C budget. The above hypotheses will be tested by employing and further developing methodology that combines multiple labelling of microorganisms with isotopic tracers and flow cytometric sorting, in combination with nutrient bioassay experiments.

该提案的目的是解释地球最大的生态系统的贫营养海洋Gyres的功能，这对全球生物地球化学和气候产生了深远的影响。与C和N的复杂生物地球化学动力学及其多个无机池相比，这些池不允许其完整的微生物预算在实验上准确确定，在地表水域中的磷酸（P）预算（尤其是在P耗尽的北大西洋Gyre）中，它易于量化，因为Inorganic P仅在其溶解形式的形式中被列出，因此它是由Microbes征服的。 The project proposes to experimentally test and to numerically model a new concept of a simplest ecosystem i.e. that: mixotrophic protists control the two dominant bacterioplankton populations, Prochlorococcus cyanobacteria and the SAR11 clade, competing for depleted inorganic P. Using a combination of outlined laboratory and oceanic cruise experiments the following hypotheses will be addressed (i)综合营养的生物而不是异养生物主导少亲营水中的细菌性吞噬作用。 （ii）造成营养的生物，而不是光营养的生物或蓝细菌在贫营养水中占主导地位； （iii）原生吞噬作用，而不是细菌性衰老的死亡 /病毒裂解占据贫营养水中的营养回收； （iv）由综合营养生物控制的贫营养生态系统在P回收和C预算方面是可持续的。上述假设将通过采用和进一步开发方法来测试，该方法将微生物的多重标记与同位素示踪剂和流式细胞仪分类结合在一起，并结合营养生物测定实验。

项目成果

Efficient CO2 fixation by surface Prochlorococcus in the Atlantic Ocean.

• DOI: 10.1038/ismej.2014.56
• 发表时间: 2014-11
• 期刊: The ISME journal
• 作者: Hartmann M;Gomez-Pereira P;Grob C;Ostrowski M;Scanlan DJ;Zubkov MV
• 通讯作者: Zubkov MV

Optimized routine flow cytometric enumeration of heterotrophic flagellates using SYBR Green I

• DOI: 10.4319/lom.2011.9.329
• 发表时间: 2011-08-01
• 期刊: LIMNOLOGY AND OCEANOGRAPHY-METHODS
• 影响因子: 2.7
• 作者: Christaki, Urania;Courties, Claude;Zubkov, Mikhail V.
• 通讯作者: Zubkov, Mikhail V.

Growth and survival of Neoceratium hexacanthum and Neoceratium candelabrum under simulated nutrient-depleted conditions

模拟营养耗尽条件下 Neoceratium hexacanthum 和 Neoceratium candelabrum 的生长和存活

• DOI: 10.1093/plankt/fbt098
• 发表时间: 2014
• 期刊: Journal of Plankton Research
• 影响因子: 2.1
• 作者: Aldridge D

Assessing amino acid uptake by phototrophic nanoflagellates in nonaxenic cultures using flow cytometric sorting.

使用流式细胞术分选评估非无菌培养物中光养纳米鞭毛虫的氨基酸摄取。

• DOI: 10.1111/j.1574-6968.2009.01715.x
• 发表时间: 2009
• 期刊: FEMS microbiology letters
• 影响因子: 2.1
• 作者: Hartmann M