GLYCOSIDASES IN HUMAN HEALTH AND DISEASE

糖苷酶在人类健康和疾病中的作用

• 批准号: 7955547
• 负责人: DAVID ROSE
• 金额: $ 4.32万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955547
• 关键词: Alpha-glucosidase; Alpha-mannosidase; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Digestion; Disease; Enzymes; Funding; Glycoside Hydrolases; Golgi Apparatus; Grant; Health; Human; Institution; Intestines; Pathway interactions; Quality Control; Research; Research Personnel; Resolution; Resources; Source; Starch; Structure; United States National Institutes of Health; base; diabetic; glucosidase I; glycosylation; improved; inhibitor/antagonist; novel; protein folding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have been studying the structures and functions of glycoside hydrolases involved in human health and disease. Golgi alpha-mannosidase II is at the core of the mammalian N-glycosylation pathway, and is an anti-metastatic target. We plan to collect some complexes with novel inhibitors that have been developed based on our structural results (some of which were from CHESS data). Maltase glucoamylase is an important enzyme in intestinal starch digestion and a target for anti-diabetics. We solved the structure with CHESS data and are now collecting novel inhibitor complexes and attempting to improve the resolution. Finally, a-glucosidase I is the first enzyme in the protein folding `quality control` pathway. This is a new structure and we wil be collecting native data and possibly derivative (halide soak) data.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们一直在研究参与人类健康和疾病的糖苷水解酶的结构和功能。 Golgiα-甘露糖苷酶II是哺乳动物N-糖基化途径的核心，是抗转移性靶标。我们计划根据我们的结构结果（其中一些来自国际象棋数据）收集一些新型抑制剂的复合物。麦芽糖酶葡萄糖酰基酶是肠道淀粉消化的重要酶，也是抗糖尿病药的靶标。我们通过国际象棋数据解决了结构，现在正在收集新颖的抑制剂复合物并试图改善分辨率。最后，A-葡萄糖苷酶I是蛋白质折叠质量控制途径中的第一个酶。这是一个新的结构，我们将收集本机数据，并可能是衍生数据（卤化物浸泡）数据。