CORE--Gene Microarray

核心--基因微阵列

• 批准号: 7885387
• 负责人: Christina Harrington
• 金额: $ 11.71万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7885387
• 关键词: Biopsy; Biostatistics Shared Resource; Cancer Center Support Grant; Cell Separation; Computer software; Consultations; Core Facility; DNA Microarray Chip; Data; Data Analyses; Data Storage and Retrieval; Development; Doctor of Philosophy; Education; Gene Expression Profiling; Genes; Informatics; Institutes; Label; Laboratories; Malignant Neoplasms; Microarray Analysis; Microarray Shared Resource; Molecular Profiling; Oligonucleotide Microarrays; Performance; Preparation; Procedures; Process; Protocols documentation; Quality Control; RNA; Research; Research Infrastructure; Research Personnel; Resource Sharing; Sampling; Services; Sister; Spottings; System; Technology; Testing; Time; Training and Education; base; cost; data format; data management; density; design; experience; member; research study

The Gene Microarray Shared Resource (GMSR) is a full service DMA microarray facility providing OHSU Cancer Institute (Cl) investigators with high-quality expression profiling services at reasonable cost. The core facility has a full-time PhD director, a laboratory manager, two research technicians and an informaticist. Services include: 1) consultation on experiment design and sample preparation; 2) expression profiling on GeneChip oligonucleotide arrays; 3) data management and storage; 4) access to an informatics laboratory equipped with high-performance PCs and microarray analysis software; 5) support for microarray data analysis; and 6) education and training on microarray technology, data formats, and data analysis software. Data management systems are maintained through the Informatics and the Biostatistics Shared Resources, and advanced data analysis is also available through this sister Cl core. We have developed a critical mass of professionals and investigators with essential capabilities, past experience in microarray technology, and sophistication to assure appropriate design and interpretation of experiments. Our well-established infrastructure and quality-controlled procedures support the delivery of rapid, reproducible and accurate microarray data to Cl investigators. In the past three years, the GMSR has processed over 50 Affymetrix array-based projects for Cl members with a throughput of over 600 arrays. Cl members have also had access to spotted microarray services through this shared resource since its development five years ago.

基因微阵列共享资源（GMSR）是一家全方位服务的DMA微阵列设施，为OHSU癌症研究所（CL）研究人员提供高质量的表达概况服务，以合理的成本提供。核心设施有一位专职博士主任，一名实验室经理，两名研究技术人员和一名信息专家。服务包括：1）有关实验设计和样品准备的咨询； 2）对二核苷酸阵列的表达分析； 3）数据管理和存储； 4）访问配备高性能PC和微阵列分析软件的信息学实验室； 5）支持微阵列数据分析； 6）关于微阵列技术，数据格式和数据分析软件的教育和培训。通过信息学和生物统计学共享资源来维护数据管理系统，并且可以通过此信息获得高级数据分析 姐妹CL核心。我们已经开发了大量的专业人士和研究人员，具有必不可少的能力，过去的微阵列技术经验以及精致，以确保对实验的适当设计和解释。我们建立的基础设施和质量控制的程序支持将快速，可重现和准确的微阵列数据传递给CL研究者。在过去的三年中，GMSR为CL成员进行了50多个基于Affymetrix阵列的项目，具有超过600个阵列的吞吐量。自五年前开发以来，CL成员还可以通过此共享资源访问斑点的微阵列服务。