Hand-held optical probe for fluorescence imaging of breast cancer

用于乳腺癌荧光成像的手持式光学探头

• 批准号: 7127016
• 负责人: ANURADHA GODAVARTY
• 金额: $ 19.66万
• 依托单位: FLORIDA INTERNATIONAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7127016
• 关键词: 3-Dimensional; Algorithms; Animal Model; Biomedical Engineering; Brain; Brain Mapping; Brain imaging; Breast; Caliber; Cancer Diagnostics; Cancer Etiology; Cancer Prognosis; Charge; Clinic; Clinical; Collection; Contrast Media; Coupled; Data; Death Rate; Depth; Detection; Development; Device or Instrument Development; Devices; Diagnostic; Diagnostic Imaging; Disease; Early Diagnosis; Elements; Epilepsy; Fiber; Florida; Fluorescence; Frequencies; Future; Hand; Image; Imaging Techniques; In Vitro; Institutes; Intensive Care Units; International; Invasive; Lead; Light; Lighting; Mammary Gland Parenchyma; Measurement; Modality; Monitor; Nature; Noise; Numbers; Optics; Patients; Pediatric Hospitals; Pliability; Population; Rate; Research; Research Personnel; Resolution; Scheme; Sensitivity and Specificity; Shapes; Signal Transduction; Staging; Students; System; Technology; Time; Tissue Model; Tissue Sample; Tissues; Translations; United States; Universities; Work; absorption; base; cancer diagnosis; cancer imaging; clinically relevant; data acquisition; design; design and construction; fluorescence imaging; improved; in vivo; innovation; instrument; malignant breast neoplasm; novel; optical fiber; optical imaging; outcome forecast; portability; prognostic; programs; tissue phantom; tomography; tool

DESCRIPTION (provided by applicant): Diagnostic imaging of early stage breast cancer is essential for decreasing the death rate caused by cancer in the United States. Near-infrared (NIR) optical imaging using external fluorescence contrast agents is an emerging non-invasive modality that can become an important tool in the diagnosis of early-stage breast cancer and prognosis of the disease. To date, most of the work in fluorescence-enhanced optical tomographic imaging is carried out using: (i) small volume phantom or in-vivo animal models that lack clinically relevant depth information; (ii) non-flexible optical probes that are restricted to image only specific tissue volumes or shapes; or (iii) measurement geometries that interrogate limited tissue volumes. In the current application, we propose to develop a hand-held optical fiber probe (~ 2" diameter) to perform fluorescence imaging of breast cancer using large tissue phantoms and in-vitro tissue models. The hand- held optical probe will be unique in terms of its: (i) flexibility to image any tissue shape and volume; (ii) non- compressibility, portability, and patient comfort due to its hand-held based design; and (iii) novel measurement geometry that can interrogate greater tissue volumes with reduced imaging time. The developmental aims are to: (i) design and construction of a hand-held optical with optimal fiber configuration for simultaneous NIR light illumination and collection measurement geometry; (ii) develop and optimize a rapid data acquiring frequency-domain intensified charge coupled device (ICCD) detection system; (iii) perform fluorescence-enhanced absorption imaging studies using tissue-mimicking phantoms and in-vitro tissue samples and assess the precision and accuracy of the fluorescence measurements; and (iv) integrate an undergraduate student with current research at various stages of the proposed work. The milestone of the proposed work is to develop a hand-held optical-based imagerthat is robust, rapid, flexible, non- compressible, comfortable, portable, and applicable for any given breast volume and shape, that is expected to render high-resolution diagnostic and prognostic breast cancer imaging in the clinic.

描述（由申请人提供）：早期乳腺癌的诊断成像对于降低美国癌症死亡率至关重要。使用外部荧光造影剂的近红外（NIR）光学成像是一种新兴的非侵入性方式，可以成为诊断早期乳腺癌和疾病预后的重要工具。迄今为止，荧光增强光学断层成像的大部分工作都是使用：（i）缺乏临床相关深度信息的小体积体模或体内动物模型； (ii) 非柔性光学探头，仅限于对特定组织体积或形状进行成像； (iii) 询问有限组织体积的测量几何形状。在目前的应用中，我们建议开发一种手持式光纤探头（直径约2英寸），利用大型组织模型和体外组织模型对乳腺癌进行荧光成像。该手持式光纤探头将在以下领域中独一无二：其优势在于：(i) 可对任何组织形状和体积进行成像；(ii) 由于其手持式设计，具有不可压缩性、便携性和患者舒适度；(iii) 新颖的测量几何结构，可以检测更大的组织体积。减少成像时间。开发目标是：（i）设计和构建具有最佳光纤配置的手持式光学器件，用于同步近红外光照明和收集测量几何结构；（ii）开发和优化快速数据采集频域增强电荷耦合器件（ICCD）； ) 检测系统；(iii) 使用模拟组织模型和体外组织样本进行荧光增强吸收成像研究，并评估荧光测量的精度和准确度； (iv) 将本科生与拟议工作的各个阶段的当前研究相结合。这项工作的里程碑是开发一种手持式光学成像仪，该成像仪坚固、快速、灵活、不可压缩、舒适、便携，适用于任何给定的乳房体积和形状，有望提供高分辨率临床中的诊断和预后乳腺癌成像。