Rhizo-mining of root exudates for therapeutics against Enterococcus species

根部分泌物的根部开采用于治疗肠球菌属物种

基本信息

批准号：
7898572
负责人：
Harsh P. Bais
金额：
$ 17.52万
依托单位：
UNIVERSITY OF DELAWARE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-23 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7898572
关键词：
Agar Age Animal Model Anti-Infective Agents Antibiotics Antimicrobial Effect Applications Grants Attenuated Bacteria Biological Assay Biological Models Caenorhabditis elegans Cessation of life Characteristics Chemicals Coculture Techniques Colony-forming units Communication Data Defense Mechanisms Development Diffusion Disease Effectiveness Enterococcus Enterococcus faecalis Environment Escherichia coli Exhibits Exudate Genes Genetic Screening Genome Goals Grant Growth Hospitals Human Immunity In Vitro Infection Intestines Investigation Laboratories Lead Left Life Measures Methods Microbe Minimum Inhibitory Concentration measurement Mining Modeling Molecular Weight Nematoda Nematode infections Nosocomial Infections Organ Organism Pathogenesis Pathogenicity Pathway interactions Phenotype Plant Model Plant Roots Plants Play Principal Investigator Property Pseudomonas Pseudomonas aeruginosa Publishing Research Research Design Resistance Role Screening procedure Serine Protease Signal Transduction Soil Source Sterility Stimulus Stress Study Section Symptoms System Systemic infection Testing Therapeutic Therapeutic Agents United States National Institutes of Health Virulence Virulence Factors Work abstracting antimicrobial bactericide base chemical genetics drug discovery factor C frontier insight interest mutant novel novel strategies novel therapeutics pathogen product development programs quorum sensing

项目摘要

Program Director/Principal Investigator (Last, First, Middle): Bais, Harsh P. 1 R21 AI073323-01A1 ABSTRACT The research outlined in this grant proposes the development of an alternative therapeutic approach to treat Enterococcus infections. An important characteristic of this genus of bacteria is their resistance towards chemical and physical stress also, in contrast to other faecal bacteria that are released into the environment e.g. Escherichia coli, E. faecalis and E. faecium can survive for long periods outside their natural intestinal hosts. The E. faecalis and E. faecium are frequently associated with nosocomial infections (i.e. acquired in the hospital setting) and are resistant to almost all of the modern-age antibiotics. The proposed screening model involving plant root exudates presents a novel source for isolation of compounds that attenuate E. faecalis' and E. faecium's virulence and could potentially serve as an alternative approach to treat E. faecalis and E. faecium infections by discovering novel anti-infectives. The specific aims are Aim 1: To apply a whole plant screening method to test Enterococcus species pathogenicity. Identify plant species that reveal a tolerant phenotype in the in vitro based infections. Characterize the root secretions of the tolerant species against Enterococcus species. Test the anti-infective (virulence factors) activity of the tolerant root exudates in the nematode assays. Aim 2: To characterize compound(s) from the root exudates of tolerant plant species that exhibit antiinfective properties against Enterococcus species. To use classical nematode infection assays to characterize the effectiveness of the identified compound as an anti-infective agent. It is expected that these novel efforts will lead to the development of new therapeutics leading to characterization of anti-infective for the treatment of Enterococcus infections.

项目总监/首席研究员（最后，第一，中间）：Bais，严苛 P.1 R21 AI073323-01A1 抽象的这笔赠款中概述的研究建议开发一种治疗肠球菌感染的替代治疗方法。该细菌属的一个重要特征是它们对化学和物理压力的抵抗力，与释放到环境中的其他粪便细菌（例如粪便细菌）相反。大肠杆菌、粪肠球菌和屎肠球菌可以在其天然肠道宿主之外存活很长时间。粪肠球菌和屎肠球菌经常与医院感染（即在医院环境中获得）相关，并且对几乎所有现代抗生素具有耐药性。所提出的涉及植物根分泌物的筛选模型为分离可减弱粪肠球菌和屎肠球菌毒力的化合物提供了新的来源，并可能通过发现新的抗-粪肠球菌和屎肠球菌感染作为治疗粪肠球菌和屎肠球菌感染的替代方法。感染者。具体目标是目标1：应用全株筛选方法来测试肠球菌种致病性。鉴定在体外感染中表现出耐受表型的植物物种。表征耐肠球菌属物种的根分泌物。在线虫测定中测试耐受根分泌物的抗感染（毒力因子）活性。目标 2：表征具有抗肠球菌属物种抗感染特性的耐受植物物种根部分泌物中的化合物。使用经典的线虫感染测定来表征所鉴定的化合物作为抗感染剂的有效性。预计这些新的努力将导致新疗法的开发，从而确定用于治疗肠球菌感染的抗感染剂的特性。