AR COBRE: MEMBRANE PROTEINS

AR COBRE：膜蛋白

• 批准号: 7959346
• 负责人: denise greathouse
• 金额: $ 27.76万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959346
• 关键词: Address; Binding; Biochemistry; Biological; Biological Models; Biological Process; Cell physiology; Centers of Research Excellence; Clinical Medicine; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytochrome P450; Development; Diagnostic; Enzymes; Fluorescence Microscopy; Funding; Goals; Grant; Healthcare; Institution; Intervention; Investigation; Learning; Lipids; Medical; Membrane; Membrane Biology; Membrane Proteins; Methods; Modeling; NMR Spectroscopy; Proteins; Reaction; Regulation; Research; Research Personnel; Resources; Science; Source; Staging; System; Techniques; Tertiary Protein Structure; United States National Institutes of Health; base; design; frontier; insight; protein function; protein structure function; prototype; solid state nuclear magnetic resonance

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biophysical investigations are proposed to elucidate key aspects of the functional interactions between proteins and lipids in biological membranes. Membrane proteins are an important frontier within not only biochemistry, but science generally. Membrane protein function has crucial implications for cell physiology and the regulation of biological function. A key goal of this project will be to develop and advance experimental methods in solid-state NMR spectroscopy and fluorescence microscopy that are needed for addressing the mechanisms by which proteins and lipids interact within biological membranes. Due to the complexity of biological membranes, the projects proposed here will, initially, make use of judiciously designed model systems that consist of small, hydrophobic protein domains and that will pave the way for studies of more complex systems. We then will extend the methods to investigate and characterize a prototype system of greater complexity, the membrane-anchored cytochrome P450 enzyme. The model systems provide two means for setting the stage: they allow understanding and insight into fundamental molecular interactions; and they lend themselves toward the development of experimental techniques that then become available for application to other (larger) systems, such as the complexes of P450 with membrane-bound reaction partners, and beyond. Tangible benefits for health care may accrue in two ways: The model and prototype systems themselves may suggest new strategies for membrane-based interventions in diagnostic or clinical medicine. Furthermore, the general membrane principles that are learned may be applied to medical issues in membrane biology.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 提出了生物物理研究，以阐明生物膜中蛋白质与脂质之间功能相互作用的关键方面。 膜蛋白不仅是生物化学的重要边界，而且是科学的一般领域。 膜蛋白功能对细胞生理和生物功能的调节具有至关重要的影响。 该项目的一个关键目标是在固态NMR光谱和荧光显微镜中开发和推进实验方法，这些方法是解决蛋白质和脂质在生物膜中相互作用的机制所需的。 由于生物膜的复杂性，此处提出的项目最初将利用由小型的疏水蛋白领域组成的明智设计的模型系统，这将为更复杂的系统的研究铺平道路。 然后，我们将扩展研究和表征具有更大复杂性的原型系统，即膜锚定的细胞色素P450酶。 模型系统提供了两种设定阶段的手段：它们允许理解和洞悉基本分子相互作用；它们倾向于开发实验技术，然后将其用于其他（较大）系统，例如p450与膜结合的反应伙伴以及其他伴侣的配合物。 医疗保健的切实益处可以通过两种方式产生：模型和原型系统本身可能提出了基于膜或临床医学的基于膜干预措施的新策略。 此外，所学的一般膜原理可以应用于膜生物学的医学问题。