PRION STRAIN TARGETING AND COMPETITION IN THE CENTRAL NERVOUS SYSTEM

中枢神经系统中朊病毒株的靶向和竞争

• 批准号: 7959390
• 负责人: Jason C Bartz
• 金额: $ 6.49万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959390
• 关键词: Area; Brain; Computer Retrieval of Information on Scientific Projects Database; Funding; Goals; Grant; Hamsters; Infection; Institution; Integration Host Factors; Investigation; Lead; Location; Modeling; Motor; Nebraska; Neuraxis; Neurons; Prion Diseases; Prions; Research; Research Personnel; Resources; Rodent; Source; Testing; United States National Institutes of Health; cell type; sciatic nerve; spinal tract; virology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this proposal is to identify the effects of prion infection on replication of a subsequently inoculated second prion strain. Prior inoculation of rodents with a long incubation prion strain can delay the incubation period or block a subsequently inoculated (i.e. superinfected) short incubation prion strain, however, the location and cell types in the CNS where prion strain selection occur are not known. Investigation of the mechanisms responsible for the inhibition of the superinfected prion strain replication may lead to identification of host factors involved in prion replication. The hypothesis to be tested is that prion infection of neurons inhibits subsequent prion infection and/or replication of another prion strain. To test this hypothesis, the sciatic nerve model of prion strain targeting and competition will be utilized. In this model of prion disease, prions are transported to the brain along three motor spinal tracts. Inoculation of the sciatic nerve with the long incubation period drowsy strain of hamster-adapted TME (DY TME) 120 days prior to sciatic nerve inoculation with the short incubation period hyper TME (HY TME) strain can block HY TME and results in DY TME. The sciatic nerve model of prion strain selection can facilitate the identification of the areas and cell types involved in prion strain selection.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该提案的目的是确定病毒感染对随后接种第二个prion菌株的复制的影响。 事先接种长期孵育菌株的啮齿动物会延迟孵化周期，或者随后接种（即超染料）短孵育prion菌株的A菌株，但是，在CNS中选择prion菌株的位置和细胞类型尚不清楚。 调查负责抑制超级感染的Prion菌株复制的机制可能导致鉴定涉及prion复制的宿主因素。 要检验的假设是神经元的prion感染抑制了随后的prion感染和/或复制另一个prion菌株。 为了检验这一假设，将利用Prion菌株靶向和竞争的坐骨神经模型。 在这种prion疾病模型中，沿三个运动脊柱传输到大脑。 在坐骨神经接种前120天，与仓鼠适应的TME（DY TME）的长期孵育周期接种坐骨神经，drolly weartial菌株，坐骨神经接种前120天，短期孵育期Hy-TME（hy TME）菌株可以阻止TME，并在DY TME中阻止TME和结果。 prion菌株选择的坐骨神经模型可以促进鉴定涉及prion菌株的区域和细胞类型。