SIGNAL TRANSDUCTION CORE

信号传导核心

• 批准号: 7959696
• 负责人: CHRISTOPHE P NICOT
• 金额: $ 7.17万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959696
• 关键词: Cells; Characteristics; Charge; Computer Retrieval of Information on Scientific Projects Database; Computer software; Equipment; Funding; Grant; Image; Institution; Kinetics; Life; Microscope; Photography; Property; Proteins; Research; Research Personnel; Resources; Signal Pathway; Signal Transduction; Source; System; Time; United States National Institutes of Health; cellular imaging; cellular transduction; design; interest; microbial; novel strategies; pathogen; single molecule; Cells; Characteristics; Charge; Computer Retrieval of Information on Scientific Projects Database; Computer software; Equipment; Funding; Grant; Image; Institution; Kinetics; Life; Microscope; Photography; Property; Proteins; Research; Research Personnel; Resources; Signal Pathway; Signal Transduction; Source; System; Time; United States National Institutes of Health; cellular imaging; cellular transduction; design; interest; microbial; novel strategies; pathogen; single molecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this core is to provide access to an epifluorescence microscope with characteristics of a confocal. Technical assistance in the design and utilization of the equipment is (currently) provided free of charge to investigators interested in studying cellular transduction and signaling pathways. Observations from studies with fluorescently tagged proteins typically show the steady state distribution of a protein, but they do not directly provide information about the kinetic properties of molecules. Acquisition of a Nikon single molecule imaging system equipped for time lapse photography of single cells while under incubation at 37 degrees C has been purchased and is now fully equipped with Metamorph software and functional for live cell imaging studies.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该核心的目的是提供具有共焦特征的落荧光显微镜的访问。设备设计和利用方面的技术援助（当前）是为有兴趣研究细胞转导和信号通路的调查人员免费提供的。 对荧光标记蛋白的研究的观察结果通常显示出蛋白质的稳态分布，但它们并未直接提供有关分子动力学特性的信息。已经购买了在37摄氏度孵育的同时，采集配备了单个细胞的尼康单分子成像系统，现已购买，现在完全配备了Metamorph软件，并且功能性用于实时细胞成像研究。