Whole Mouse Cryo-Imaging

全小鼠冷冻成像

基本信息

批准号：
7940008
负责人：
DAVID Lynn WILSON
金额：
$ 34.91万
依托单位：
BIOINVISION, INC.
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7940008
关键词：
Anatomy Animals Area Automation Beds Biomedical Engineering Biomedical Research Biotechnology Businesses Cancer Biology Cancer cell line Cells Cellular Structures Color Communication Computer software Computers Crying Data Data Set Data Storage and Retrieval Development Devices Disease Disseminated Malignant Neoplasm Drug Delivery Systems Ensure Equilibrium Face Fee-for-Service Plans Fluorescence Freezing Gene Expression Glossary Green Fluorescent Proteins Heating Hematoxylin and Eosin Staining Method Histology Ice Image Image Analysis Imagery Immune system Industry Injection of therapeutic agent Intervention Label Language Learning Lewis Lung Carcinoma Light Lighting Liver Local Area Networks Location Lung Magnetic Resonance Imaging Malignant Neoplasms Maps Marketing Measurement Measures Mechanics Medicine Metadata Methods Microscopic Microscopy Microtus Modality Modeling Molecular Mus Neurosciences Noise Operative Surgical Procedures Optics Organ Organ Size Phase Phenotype Positioning Attribute Positron-Emission Tomography Preparation Process Protein Region Protocols documentation Regenerative Medicine Relative (related person)Reporter Genes Research Research Personnel Resolution Robotics Scheme Site Source Specimen Speed Staging Staining method Stains Stem cells Structure Surface System Tail Techniques Temperature Testing Therapeutic Three-Dimensional Imaging Time Tissue Engineering Tissues Transgenic Organisms Universities Vacuum Validation Veins Writing X-Ray Computed Tomography biological research cancer cell cancer therapy cryostat data acquisition digital imaging experience fluorescence imaging fluorescence microscope formamide gene therapy graphical user interface imaging Segmentation improved in vivo interest meetings molecular domain molecular/cellular imaging next generation pathogen programs prototype research study response sensor stem cell biology stem cell therapy tomography ultra high resolution vibration

项目摘要

DESCRIPTION (provided by applicant): BioInVision, Inc. and Case Western Reserve University researchers will develop a cryo-imaging system for 3D imaging of mice. It will serve very many applications in biological research and the biotechnology industry, including mouse anatomical phenotyping, drug delivery, imaging agents, metastastic cancer, pathogen/immune system response, and stem cells/regenerative medicine, as well as many other studies of disease and therapeutic processes. By alternately sectioning and imaging, the system will acquire 3D, high-resolution anatomical color and cellular and molecular fluorescence images. The cryo-imaging system will consist of a mouse-sized, motorized cryostat with special features for imaging, a modified, bright-field/fluorescence microscope, and a robotic xyz imaging system positioner, all of which will be fully automated by a control system. The system will image fluorescent-reporter-gene labeled cells. By imaging with high resolution and sensitivity, we will establish a method for identifying single cells or cell clusters within a mouse. Once cells are identified, cell locations can be mapped relative to the tissue anatomy in the high contrast, 3D cryo-image color volumes. Cryo-imaging is unique among all in vivo and microscopic techniques in that it allows micron resolution and information-rich contrast mechanisms over very large 3D fields of view. It will fill the gap between whole animal in vivo imaging and histology, allowing one to image a mouse along the continuum from the mouse -> organ -> tissue structure -> cell -> sub-cellular domains.

描述（由申请人提供）：BioInVision, Inc. 和凯斯西储大学的研究人员将开发一种用于小鼠 3D 成像的冷冻成像系统。它将在生物研究和生物技术行业中有很多应用，包括小鼠解剖表型、药物输送、显像剂、转移性癌症、病原体/免疫系统反应、干细胞/再生医学，以及许多其他疾病和疾病的研究。治疗过程。通过交替切片和成像，系统将获取 3D、高分辨率解剖彩色图像以及细胞和分子荧光图像。冷冻成像系统将包括一个鼠标大小的、具有成像特殊功能的电动低温恒温器、一个改进的明场/荧光显微镜和一个机器人 xyz 成像系统定位器，所有这些都将由控制系统完全自动化。该系统将对荧光报告基因标记的细胞进行成像。通过高分辨率和高灵敏度的成像，我们将建立一种识别小鼠体内单细胞或细胞簇的方法。一旦识别出细胞，就可以在高对比度 3D 冷冻图像彩色体积中相对于组织解剖结构绘制细胞位置。冷冻成像在所有活体和显微技术中是独一无二的，因为它可以在非常大的 3D 视场上实现微米级分辨率和信息丰富的对比机制。它将填补整个动物体内成像和组织学之间的空白，使人们能够沿着从小鼠 -> 器官 -> 组织结构 -> 细胞 -> 亚细胞域的连续体对小鼠进行成像。