Differentiation of taste papilla epithelium during development

发育过程中味乳头上皮的分化

• 批准号: 7798017
• 负责人: Hongxiang Liu
• 金额: $ 15.03万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-31 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7798017
• 关键词: Afferent Neurons; Apical; Appearance; Area; Attention; Biology; Cell Differentiation process; Cell Lineage; Cell Maintenance; Cells; Data; Derivation procedure; Development; Embryo; Epithelial Cells; Epithelium; Esthesia; Event; Feeding behaviors; Fiber; Foundations; Fungiform Papilla; Future; Goals; Grant; Housing; Immunohistochemistry; In Vitro; Indium; Knowledge; Label; Laboratories; LacZ Genes; Life; Location; Mesenchyme; Mus; Nature; Neural Crest; Neural Crest Cell; Neurons; Newborn Infant; Organ; Organ Culture Techniques; Peripheral; Phenotype; Publishing; Quality of life; Rattus; Reporter; Rodent; Sensory; Sensory Ganglia; Signal Transduction; Staging; Stimulus; System; Taste Bud Cell; Taste Buds; Taste Perception; Time; Tongue; Transgenes; Transgenic Mice; Tubulin; Vertebrates; base; blastomere structure; cell type; density; immunoreaction; insight; postnatal; precursor cell; public health relevance; relating to nervous system; research study; tongue papilla

DESCRIPTION (provided by applicant): Taste buds, the sensory end organs that transduce gustatory stimuli into neural signals, reside in taste papillae in the mammalian tongue. Therefore, taste papillae incorporate the epithelium that will differentiate to include cells of taste bud. However, the field of taste biology lacks a clear understanding of what constitutes possible taste cell precursors in developing papillae. In fact, there is no detailed information about cell specification of the early lingual epithelium that can cross-signal to underlying mesenchyme in papilla differentiation. The goals of this proposal are to characterize: (1) the localization and timing for neuronal, glial and taste cell phenotypes in embryonic and early postnatal tongue epithelium; (2) stage and location-specific distribution and phenotypic specification of neural crest derived cells (NCDCs) in developing lingual epithelium, mesenchyme and taste papillae. Immunohistochemistry on whole tongue and sections will be used to characterize and localize neuronal, glial and taste cell phenotypes, in embryonic and postnatal tongue epithelium and mesenchyme. Also, an in vitro, tongue organ culture system at select embryonic stages will be used to study whether cell types can develop and be sustained in the absence of an intact sensory contribution. We will use transgenic mouse lines with a lacZ (R26R) reporter or ZEG (lacZ/EGFP) double reporter transgene modulated by Wnt1- Cre or P0-Cre to label Wnt1- and P0-expressing NCDCs. The distribution and cell phenotypes of NCDCs in the developing tongue and taste papillae will be characterized. The results will bring new information to the field and provide an important foundation for understanding the derivation and progressive differentiation of developing taste papilla epithelium. Taste sensation, which is tranduced by taste buds in the gustatory papillae, guides nutritive choices and ingestive behaviors of all vertebrates. Thus a thorough knowledge of how taste papillae develop and differentiate to eventually include taste bud cells will contribute to better understanding how taste sensation develops. Public Health Relevance: The sense of taste informs our nutritive choices, which are essential for life and quality of life. Knowledge of taste papilla development will contribute to understanding how the distribution and density of tongue taste organs are determined, and therefore, to understanding how taste sensitivity emerges.

描述（由申请人提供）：味蕾，将味觉刺激转移到神经信号的感觉端器官，驻留在哺乳动物舌头的味道乳头上。因此，味觉乳头融合了上皮，该上皮将分化为包括味蕾细胞。然而，味觉生物学领域缺乏清楚地了解开发乳头状中可能的味觉细胞前体。实际上，没有关于早期舌上皮细胞规范的详细信息，这些信息可以交叉信号到乳头分化中的间质。该提案的目标是表征：（1）胚胎和早期产后舌头上皮中神经元，神经胶质和味觉细胞表型的定位和时间； （2）神经rest衍生细胞（NCDC）的阶段和位置特异性分布和表型规范在发展舌上皮，间质和味觉乳头状中。整个舌头上的免疫组织化学将用于表征和定位神经元，神经胶质和味觉细胞表型，在胚胎和产后舌舌上皮和间质中。此外，在某些胚胎阶段的体外，舌器官培养系统将用于研究细胞类型是否可以在没有完整的感觉贡献的情况下发展和维持。我们将使用带有LACZ（R26R）报告基因或ZEG（LACZ/EGFP）双向报告基因转基因的转基因小鼠线，由Wnt1- CRE或P0-CRE调制，以标记Wnt1-和P0表达NCDC的标签。 NCDC在发育中的舌头和味觉乳头状中的分布和细胞表型将被表征。结果将为领域带来新的信息，并为理解发展味觉乳头上皮的推导和逐步差异提供了重要的基础。味觉被味觉乳头状的味蕾所扭曲，引导了所有脊椎动物的营养选择和摄取行为。因此，对味觉乳头的发展和区分以最终包括味蕾细胞的详尽了解将有助于更好地了解味觉如何发展。 公共卫生相关性：味觉使我们的营养选择介绍，这对生活和生活质量至关重要。对口味乳头发展的了解将有助于了解如何确定舌头口味器官的分布和密度，从而了解味道敏感性的出现。