Stem Cell Core

干细胞核心

基本信息

批准号：
7806064
负责人：
MICHAEL Alan LAFLAMME
金额：
$ 28.61万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2015-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7806064
关键词：
Animals Attention Canis familiaris Cardiac Cardiac Myocytes Cardiovascular system Cause of Death Cell Culture Techniques Cell Therapy Cells Chromosomes Complex Conditioned Culture Media Data Derivation procedure Development Ensure Gene Transfer Techniques Generations Genes Genetic Goals Human Human Resources In Vitro Individual Marrow Mesenchymal Stem Cells Methods Modification Myocardial Infarction Phenotype Plasmids Pluripotent Stem Cells Preparation Production Protocols documentation Quality Control Reporter Reporting Research Personnel Stem cells Stromal Cells Sum System Testing Training Training Programs Transfection Translating Transplantation Undifferentiated Vascular Endothelial Growth Factor Receptor-2 Work base cell type cost embryonic stem cell experience human embryonic stem cell in vivo induced pluripotent stem cell large scale production progenitor programs repaired research study scale up tool

项目摘要

Core A: While pluripotent stem cells and their differentiated progeny have tremendous promise for cardiac repair, their routine culture is demanding and requires considerable expertise and continuous attention to quality control. Based on our extensive prior experience with these cells, we have found that centralization of cell culture efforts is the best way to ensure consistency and efficiency in the large-scale production of undifferentiated and differentiated cells. Hence, the primary goal ofthe Stem Cell Core will be to generate large numbers of undifferentiated embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs) and cardiac derivatives for distribution to the individual projects in this program. The Core will also work closely with investigators in Project 2 to optimize the derivation of cardiomyocytes from canine ESCs. Finally, the Core will also develop and share optimized protocols for the genetic modification of ESCs and iPSCs, including the generation of cell type specific reporter lines via transgenesis in bacterial artficial chromosomes (BACs). In sum, the Core will undertake three specific aims: 1) to provide the individual projects with the stem cells and cardiac derivatives needed to complete their respective aims, 2) to develop BAC transgenesis and optimize the delivery of modified BACs into human ESCs and iPSCs), and 3) to provide training and technical assistance with in vitro experiments involving pluripotent stem cells or their differentiated progeny.

核心 A：虽然多能干细胞及其分化后代对于心脏疾病有着巨大的前景修复，他们的日常文化要求很高，需要大量的专业知识和持续的关注质量控制。根据我们之前对这些单元的丰富经验，我们发现集中化细胞培养工作是确保大规模生产的一致性和效率的最佳途径未分化和分化细胞。因此，干细胞核心的主要目标是产生大量未分化胚胎干细胞（ESC）、诱导多能干细胞（iPSC）、间充质干细胞 (MSC) 和心脏衍生物，用于分发到本项目中的各个项目程序。核心还将与项目 2 的研究人员密切合作，以优化来自犬 ESC 的心肌细胞。最后，核心还将开发和共享优化协议 ESC 和 iPSC 的遗传修饰，包括通过生成细胞类型特异性报告系细菌人工染色体（BAC）转基因。总之，核心将实现三个具体目标： 1) 为各个项目提供完成其项目所需的干细胞和心脏衍生物各自的目标，2) 开发 BAC 转基因并优化将修饰的 BAC 递送至人体 ESC 和 iPSC），以及 3）提供涉及体外实验的培训和技术援助多能干细胞或其分化后代。