MUTANT MOUSE RESOURCE AND RESEARCH CENTER: AIDS

突变小鼠资源和研究中心：艾滋病

• 批准号: 8173529
• 负责人: JOHN K. CRITSER
• 金额: $ 29.01万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173529
• 关键词: Acquired Immunodeficiency Syndrome; Archives; Arts; Assisted Reproductive Technology; Biomedical Research; Biosensor; Cells; Communities; Computer Retrieval of Information on Scientific Projects Database; Congenic Strain; Cryopreservation; Development; Disease; Embryo; Funding; Genetic; Genetically Engineered Mouse; Goals; Grant; Growth; Health; Human; Injection of therapeutic agent; Institution; Intracytoplasmic Sperm Injections; Methodology; Missouri; Modeling; Mus; Mutant Strains Mice; Ovarian Tissue; Pathogen detection; Policies; Research; Research Personnel; Research Project Grants; Resources; Services; Source; Speed; Technology; Tissue Transplantation; United States National Institutes of Health; base; cost; embryonic stem cell; improved; new technology; nuclear transfer; operation; repository

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): The importance of genetically-engineered mice (GEM) as models of human health and disease has been demonstrated unequivocally. Because of the importance of GEM in biomedical research, the numbers of GEM lines have grown exponentially over the last five years; this astronomical growth is predicted to continue. Recognizing the scientific importance of sharing mice among investigators, the NIH developed a mouse sharing policy which states that mouse lines generated with the aid of NIH funding must be distributed and shared with the scientific community. To accomplish this goal, the NIH funded the establishment of four regional Mutant Mouse Resource and Research Centers (MMRRCs) in 1999 and 2000. This application is a competitive renewal for the Missouri/Harlan (M/H) MMRRC. Specific Aims of the proposal are: (1) To improve the efficiencies of the MMRRC service functions while continuing to provide the scientific community with a mutant mouse repository and distribution center of the highest quality. To accomplish this, we will employ state-of-the-art cryopreservation and reanimation (reestablishment of lines after cryopreservation) technologies, such as intracytoplasmic sperm injection (ICSI), nuclear transfer (NT) ovarian tissue (OT) transplantation (OTT) and injection of NT derived embryonic stem cells (ntES cells) into tetraploid embryos (ntES/4N). Using these approaches we will be able to more efficiently import, cryopreserve and reanimate lines at a cost approximately 10% of current, traditional embryo cryopreservation based approaches. (2) To conduct research aimed at increasing the value of the MMRRC. Research will include projects aimed at improving assisted reproductive technologies (ARTs) used for reanimation of mouse lines, development of biosensors for detection of pathogens and health surveillance of mutant mouse colonies, and development of new genetic and ARTs that will revolutionize the speed and efficiency with which congenic strains of mice are made. The proposed approach to the operation of the M/H MMRRC represents a new paradigm for mouse repositories. Recognizing the continued exponential growth of GEM, a new paradigm is mandatory; existing mouse resource centers simply cannot archive all of the important mouse lines using traditional methodology. Furthermore, the proposed research projects will result in even greater efficiencies for the MMRRC and the development of new technologies useful to the entire scientific community.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 关于艾滋病 描述（由申请人提供）： 基因工程小鼠（GEM）作为人类健康和疾病模型的重要性已被明确证明。由于宝石在生物医学研究中的重要性，在过去的五年中，宝石系的数量呈指数增长。预计这种天文生长将继续。 NIH认识到在调查人员之间共享小鼠的科学重要性，因此制定了一项鼠标共享政策，该政策指出，必须在NIH资金的帮助下分配并与科学界共享鼠标线条。为了实现这一目标，NIH资助了1999年和2000年建立四个区域突变的小鼠资源和研究中心（MMRRC）。此应用程序是密苏里州/Harlan（M/H）MMRRC的竞争更新。该提案的具体目的是：（1）提高MMRRC服务功能的效率，同时继续为科学界提供最高质量的突变小鼠存储库和分配中心。 To accomplish this, we will employ state-of-the-art cryopreservation and reanimation (reestablishment of lines after cryopreservation) technologies, such as intracytoplasmic sperm injection (ICSI), nuclear transfer (NT) ovarian tissue (OT) transplantation (OTT) and injection of NT derived embryonic stem cells (ntES cells) into tetraploid embryos (ntES/4N).使用这些方法，我们将能够更有效地导入，冷冻保存和更新线，约占基于传统的胚胎冷冻保存方法的10％。 （2）进行旨在增加MMRRC价值的研究。研究将包括旨在改善用于复活小鼠线的辅助生殖技术（艺术）的项目，生物传感器的开发用于检测病原体和对突变小鼠菌落的健康监测，以及开发新的遗传和艺术，这些遗传和艺术将彻底改变速度和效率，从而彻底改变了小鼠的速度和效率。 M/H MMRRC操作的建议方法代表了小鼠存储库的新范式。认识到宝石的持续指数增长，必须进行新的范式。现有的鼠标资源中心根本无法使用传统方法来存档所有重要的鼠标线条。此外，拟议的研究项目将对MMRRC和对整个科学界有用的新技术的开发提高效率。