SMOOTH MUSCLE CELL ARACHIDONIC ACID RELEASE, MIGRATION, AND PROLIFERATION

平滑肌细胞花生四烯酸的释放、迁移和增殖

• 批准号: 7954012
• 负责人: Sung K. Moon
• 金额: $ 0.55万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954012
• 关键词: Arachidonic Acids; Blood Vessels; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Family; Funding; Grant; Institution; Ionophores; Lipids; Mass Spectrum Analysis; Mediating; Mus; Phospholipase A2; Process; Regulation; Research; Research Personnel; Resources; Signal Transduction; Smooth Muscle Myocytes; Source; Thapsigargin; United States National Institutes of Health; biomedical resource; member; migration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pharmacologic evidence suggests that the lipid products generated by one or more Ca2+-independent phospholipases A2 (iPLA2s) participate in the regulation of vascular tone through smooth muscle cell (SMC) Ca2+ signaling and the release of arachidonic acid. However, the recent identification of new members of the iPLA2 family, each inhibitable by (E)-6-(bromomethyl ene)-3-(1-naphthal enyl )- 2 H- t e t r a hy dr o py r a n- 2 - one, has rendered definitive identification of the specific enzyme(s) mediating these processes difficult. Accordingly, we used iPLA2b-/- mice to demonstrate that iPLA2b is responsible for the majority of thapsigargin and ionophore (A23187)-induced arachidonic acid release from SMCs.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 药理学证据表明，一种或多种不依赖 Ca2+ 的磷脂酶 A2 (iPLA2) 产生的脂质产物通过平滑肌细胞 (SMC) Ca2+ 信号传导和花生四烯酸的释放参与血管张力的调节。然而，最近鉴定出 iPLA2 家族的新成员，每个成员均可被 (E)-6-(溴亚甲基)-3-(1-萘基)- 2 H-四氢吡喃 a n- 2 - 抑制，使得对介导这些过程的特定酶的明确鉴定变得困难。因此，我们使用 iPLA2b-/- 小鼠来证明 iPLA2b 负责大部分毒胡萝卜素和离子载体 (A23187) 诱导的 SMC 中花生四烯酸的释放。