SPECIFICITY OF HISTONE ACETYLTRANSFERASE ENZYMES FROM ARABIDOPSIS THALIANA

拟南芥组蛋白乙酰转移酶的特异性

基本信息

批准号：
7953917
负责人：
CRAIG Stuart PIKAARD
金额：
$ 0.06万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-02-01 至 2010-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7953917
关键词：
Binding Proteins Biochemical Biochemistry Chromatin Computer Retrieval of Information on Scientific Projects Database Cytogenetics DNA DNA Methylation DNA Methyltransferase DNA Modification Methylases Enzymes Epigenetic Process Funding Gene Silencing Gene Targeting Genes Genetic Genetic Techniques Grant Histones Institution Mass Spectrum Analysis Modeling Molecular Biology Mouse-ear Cress Pathway interactions Plants Play RNA Interference Repression Research Research Personnel Research Project Grants Resources Role Small RNA Source Specificity United States National Institutes of Health biomedical resource chromatin modification chromatin protein histone acetyltransferase histone modification knock-down rRNA Genes

Binding Proteins Biochemical Biochemistry Chromatin Computer Retrieval of Information on Scientific Projects Database Cytogenetics DNA DNA Methylation DNA Methyltransferase DNA Modification Methylases Enzymes Epigenetic Process Funding Gene Silencing Gene Targeting Genes Genetic Genetic Techniques Grant Histones Institution Mass Spectrum Analysis Modeling Molecular Biology Mouse-ear Cress Pathway interactions Plants Play RNA Interference Repression Research Research Personnel Research Project Grants Resources Role Small RNA Source Specificity United States National Institutes of Health biomedical resource chromatin modification chromatin protein histone acetyltransferase histone modification knock-down rRNA Genes

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Pikaard lab studies the ways genes are activated and repressed, using techniques of genetics, biochemistry and molecular biology. Our current research projects are focused on gene silencing and the role that chromatin proteins and small RNAs (siRNAs) play in these epigenetic phenomena. the research focuses on plants, the important one for this project is Arabidopsis thaliana. Inactive rRNA genes are silenced via chromatin modifications. rRNA gene silencing involves concerted changes in both DNA methylation and histone modification and we have proposed a model whereby DNA and histone modifications are each upstream of one another in a self-reinforcing, circular pathway. The Pikaard group wishes to identify the chromatin modifying activities involved in this repression cycle, using RNA-interference to knock down the expression of targeted genes. Two histone deacetylases, one DNA methyltransferase and several methylcytosine binding proteins have been identified in the screens thus far. We are making use of a variety of genetic, cytogenetic and biochemical approaches in order to understand the mechanism(s) of action of these chromatin modifying activities and to understand how their actions are intertwined to comprise an epigenetic on-off switch

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。 Pikaard实验室研究了使用遗传学，生物化学和分子生物学技术激活和抑制基因的方式。我们当前的研究项目的重点是基因沉默以及染色质蛋白和小RNA（siRNA）在这些表观遗传现象中起的作用。该研究的重点是植物，该项目的重要一个是拟南芥。通过染色质修饰使非活性rRNA基因沉默。 rRNA基因沉默涉及DNA甲基化和组蛋白修饰的一致变化，我们提出了一个模型，在该模型中，DNA和组蛋白修饰在自我增强的圆形途径中彼此之间彼此之间相互上游。 Pikaard组希望使用RNA干扰来降低靶向基因的表达，以识别该抑制周期中涉及的染色质修饰活性。到目前为止，在筛选中已经鉴定出了两种组蛋白脱乙酰基酶，一种DNA甲基转移酶和几种甲基霉素结合蛋白。我们正在利用各种遗传，细胞遗传学和生化方法，以了解这些染色质修饰活性的作用机制，并了解如何交织以包含表观遗传开关的作用