INTERACTIONS OF DNA POLYMERASE WITH DNA SUBSTRATES

DNA 聚合酶与 DNA 底物的相互作用

• 批准号: 7953884
• 负责人: JOHN-STEPHEN Adolfino TAYLOR
• 金额: $ 0.23万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953884
• 关键词: Address; Adenine; Base Pairing; Computer Retrieval of Information on Scientific Projects Database; Cytosine; DNA; DNA biosynthesis; DNA-Directed DNA Polymerase; Deamination; Funding; Generations; Grant; Institution; Mass Spectrum Analysis; Mutation; Research; Research Personnel; Resources; Skin Cancer; Source; Thymine; United States National Institutes of Health; Uracil; biomedical resource; transition mutation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. C to T and CC to TT transition mutations are the most frequent mutations observed in skin cancer. A deamination hypothesis was proposed to address the generation of those mutations. In this hypothesis, a cytosine or 5-methylcytosine photoproduct deaminates to form a uracil or thymine photoproduct. Those uracil or thymine moieties in the photoproduct form Watson-Crick base-pair preferentially with adenine. After two steps of DNA replication, C to T transition mutation results.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 C至T和CC到TT过渡突变是在皮肤癌中观察到的最常见的突变。 提出了脱氨假设来解决这些突变的产生。 在这一假设中，胞嘧啶或5-甲基细胞丝光产物脱氨基形成尿嘧啶或胸腺胺光药物。 那些在光产物中的尿嘧啶或胸腺氨酸部分与腺嘌呤优先形成沃森 - 克里克碱基。 经过两步的DNA复制，C至T转变突变结果。