ISOLATION AND IDENTIFICATION OF TWO NOVEL SDS-RESISTANT SECRETED CHITINASES

两种新型抗SDS分泌型几丁质酶的分离与鉴定

• 批准号: 7954007
• 负责人: Chang Liu
• 金额: $ 0.28万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954007
• 关键词: 2-Mercaptoethanol; Aeromonas; Chitin; Chitinase; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Mass Spectrum Analysis; Measurement; Molecular Weight; N-terminal; Oligosaccharides; Proteins; Research; Research Personnel; Resistance; Resources; Soil; Source; Taiwan; United States National Institutes of Health; biomedical resource; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have isolated two SDS-resistant endochitinases, designated as ASCHI53 and ASCHI61, from Aeromonas schubertii in the soil of southern Taiwan. Mass measurement with MALDI-TOF indicates that the molecular weights are 53,527 Da for ASCHI53 and 61,202 Da for ASCHI61. N-terminal sequencing of these two chitinases suggests that thye are novel proteins. Degredation of chitin with these two endochitinases gives rise to hexameric chitin oligosaccharide, a compound known to have various potent biomedical functions. In the presence of 5% SDS, ASCHI53 and ASCHI61 retain 65% and 75% chitinase activities, respectively while the chitinolytic activities completely retain even in the presence of 10% beta-mercaptoethanol.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们分离了两个抗SDS的内胆素酶，分别从台湾南部土壤中的Aeromonas Schubertii中分离出称为Aschi53和Aschi61。 用MALDI-TOF进行质量测量表明，Aschi53的分子量为53,527 DA，而Aschi61的分子量为61,202 DA。这两个几丁质酶的N末端测序表明您是新型蛋白质。 这两个内切蛋白酶对几丁质的降解会引起六聚丁质寡糖，这是一种已知具有各种有效生物医学功能的化合物。 在存在5％SDS的情况下，Aschi53和Aschi61分别保留了65％和75％的几丁质酶活性，而在存在10％β-凝集乙醇的情况下，几丁丁蛋白分解活性也完全保留。