La Jolla Interdisciplinary Neuroscience Center Cores - Electro Core BU

拉荷亚跨学科神经科学中心核心 - Electro Core BU

基本信息

批准号：
7925713
负责人：
STUART A LIPTON
金额：
$ 9.48万
依托单位：
SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7925713
关键词：
Acetylcholine Aftercare Agar Agonist Amphotericin B Amplifiers Antibiotics Area Arts Axon Biomedical Research Blood capillaries Brain Caliber Cancer Center Support Grant Carbon Dioxide Cell membrane Cells Cerebrospinal Fluid Cities Collaborations Collagen Commit Computer software Computers Contractor Core Facility Culture Techniques Cultured Cells Data Data Analyses Data Collection Defect Development Devices Dimensions Ectopic Expression Egtazic Acid Electric Capacitance Electrodes Electrophysiology (science)Elements Excitatory Postsynaptic Potentials Experimental Designs Fiber Fluorescence Fostering Frequencies Funding Glass Glucose Glutamate Receptor Goals Grant Guanosine Triphosphate Heating Hippocampus (Brain)Horns Hour Human Resources Ice Institutes Ion Channel Island Kinetics Knockout Mice Knowledge Label Laboratories Lasers Lesion Light Lighting Link Lip structure Magnesium Chloride Measures Membrane Mesylates Methods Microelectrodes Microscope Modeling Modification Molecular Monitor Mus N-Methylaspartate Nervous system structure Neurites Neurons Neurosciences Optics Output Papain Pathway interactions Perfusion Pharmaceutical Preparations Phase Phenolsulfonphthalein Physiologic pulse Physiological Polyenes Population Preparation Probability Property Proteins Protocols documentation Pyramidal Cells Recombinant Proteins Research Research Design Research Infrastructure Research Institute Research Personnel Research Project Grants Resistance Rest Role Saline Sampling Scientist Screening procedure Series Services Side Signal Transduction Site Slice Small Interfering RNA Sodium Chloride Solutions Steel Stem cell transplant Stem cells Stimulus Stroke Sucrose Synapses Synaptic Transmission Synaptic plasticity System Techniques Temperature Testing Tetanus Tetraethylammonium Tetrodotoxin Time Transgenic Organisms Transplantation Tube Viral Vector Whole-Cell Recordings Work base capillary contactin cost desensitization design excitatory neuron extracellular flexibility heat exchanger indexing insight instrument instrumentation micromanipulator millisecond molecular imaging mutant neuronal cell body newborn neuron patch clamp postsynaptic programs receptor research study response salt balance seal tissue preparation tool vibration voltage clamp

项目摘要

ELECTROPHYSIOLOGY CORE 1. MAIN OBJECTIVES AND NEW DIRECTIONS The Electrophysiology Core will be a new facility. Currently, there is no unified Electrophysiology Core Facility to link neuroscientists on the La Jolla Torrey Pines Mesa. Therefore, this Electrophysiology facility will constitute a new Core to foster collaborations among neuroscientists in the San Diego area. Understanding how the nervous system works requires electrophysiological techniques to gain insights into how synaptic activity is regulated and modulated. The Electrophysiology Core of this Neuroscience Blueprint Initiative aims to make available electrophysiology as a research tool to a wide number of neuroscientists on the Torrey Pines Mesa. In the past several years, the Co- Directors of this Core, Dr. Stephen F. Helnemann and Dr. Charles F. Stevens made major contributions to understanding the functions of the two major excitatory transmitter systems, the nicotinic acetylcholine and glutamate receptor systems. Their work provided major insights into mechanisms of synaptic transmission and synaptic plasticity. Both researchers have contributed their knowledge and effort to a series of collaborations with neuroscientists who do not have direct expertise or the experimental set-up for electrophysiological analyses. While these collaborations accomplished significant research advancements, investigators always depended on whether the host laboratory could accommodate additional experiments in terms of instrument availability and personnel. With the increasing need for electrophysiology by developmental and systems neurobiologists, it is timely to fill the need for additional capacity in electrophysiology. To accomplish that and to further increase collaborative ventures between neuroscientists on the Torrey Pines Mesa, a new core facility for electrophysiology will be created as part of the Neuroscience Blueprint Initiative. Dr. Stephen Heinemann and Dr. Charles Stevens will serve as Co-Directors for this core and are committed to contribute to the design of electrophysiological experiments by the core users. The Electrophysiology Core has two goals: First, to provide expertise and instrumentation for electrophysiological experiments by neuroscientists at the Salk Institute, UCSD, Burnham Institute for Biomedical Research (BIMR) and The Scripps Research Institute; and second, to enhance neuroscience through dialogue and collaboration between the scientists of these institutes. The Electrophysiology Core adds a new research dimension that will provide the tools and know-how for functional and activity-dependent studies designed to acquire knowledge of the activity-dependent changes occurring in neurons either intrinsically or in response to external signals. The research projects proposed by the major users require studies of neuronal activity in brain slices and/or primary neuron cultures. Moreover, this core will inter-phase with the cores for Viral Vectors/siRNA and for Molecular Imaging cores to test fluorescently tagged wild type and mutant proteins in neuronal functions after ectopic expression in brain slices and primary cells.

电生理学核心一、主要目标和新方向电生理学核心将是一个新设施。目前，电生理学还没有统一的标准。连接拉荷亚托里松台地神经科学家的核心设施。因此，这电生理学设施将构成一个新的核心，以促进神经科学家之间的合作在圣地亚哥地区。了解神经系统如何工作需要电生理技术来获得深入了解突触活动如何受到调节和调节。电生理学的核心神经科学蓝图计划旨在将电生理学作为一种研究工具多利松台地有大量的神经科学家。在过去的几年里，联合该核心的董事 Stephen F. Helnemann 博士和 Charles F. Stevens 博士做出了重大贡献对理解两个主要兴奋性递质系统的功能的贡献烟碱乙酰胆碱和谷氨酸受体系统。他们的工作提供了重要的见解突触传递和突触可塑性的机制。两位研究人员都做出了贡献他们的知识和努力与没有直接关系的神经科学家进行了一系列合作电生理分析的专业知识或实验装置。虽然这些合作取得重大研究进展时，研究人员始终依赖于是否主机实验室可以在仪器可用性和人员。随着发育和系统对电生理学的需求不断增加对于神经生物学家来说，现在是时候满足对电生理学额外能力的需求了。到实现这一目标并进一步增加神经科学家之间的合作多利松梅萨 (Torrey Pines Mesa) 是一个新的电生理学核心设施，将作为该计划的一部分而创建神经科学蓝图倡议。 Stephen Heinemann 博士和 Charles Stevens 博士将担任该核心的联合主任致力于为电生理学的设计做出贡献核心用户的实验。电生理学核心有两个目标：首先，提供索尔克神经科学家进行电生理学实验的专业知识和仪器研究所、加州大学圣地亚哥分校、伯纳姆生物医学研究所 (BIMR) 和斯克里普斯研究所研究所;其次，通过对话与合作加强神经科学这些研究所的科学家。电生理学核心增加了一个新的研究维度，将提供工具和功能性和活动依赖性研究的专门知识，旨在获取相关知识神经元本质上或响应外部发生的活动依赖性变化信号。主要用户提出的研究项目需要研究神经元活动脑切片和/或原代神经元培养物。此外，该核心将与核心相间病毒载体/siRNA 和分子成像核心以测试荧光标记的野生型和在脑切片和原代细胞中异位表达后神经元功能中的突变蛋白。