A gene map for Biomphalaria glabrata, a S. mansoni host

曼氏沙门氏菌宿主光滑双脐螺 (Biomphalaria glabrata) 的基因图谱

基本信息

批准号：
7933299
负责人：
MATTY KNIGHT
金额：
$ 5.93万
依托单位：
BIOMEDICAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-28 至 2011-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The fundamental issue of how a schistosome survives, or is targeted for destruction, in a snail host has important implications for the epidemiology of schistosomiasis. Preceding groundwork clearly showed that genetic variations in the snail Biomphalaria glabrata play a major role in the intramolluscan development of the parasitic helminth Schistosoma mansoni. Snail stocks that were genetically selected as either parasite resistant or susceptible have provided us with valuable resources for identifying markers as well as gene(s) important in the snail/parasite relationship. Our objective is to use well-established molecular tools, studying defined snail stocks with stable but different susceptibility phenotypes, to identify and characterize those snail genes that play a critical role in resisting, or fully supporting, a schistosome infection. Polymorphisms in resistant vs. susceptible snail DNA should enable us to efficiently isolate probes targeting those sequences involved in the snail/parasite relationship. Use will also be made of examining the heritability of polymorphic markers in progeny snails that segregate for resistance or susceptibility. By generating suppression subtraction hybridization (SSH) libraries from exposed vs normal resistant and susceptible juvenile snails and, by screening an available snail microarray with cDNA probes from resistant and susceptible juvenile snails ( parasite infection), we hope to identify genes that govern these different infection phenotypes in this snail-host. For positional mapping of B. glabrata chromosomes by FISH, we will use (as probes) BAG clones, corresponding to transcripts identified from the microarray/subtraction studies. Our overall strategy is to reduce the inflow of repetitive markers and combine useful information gained at both DNA and RNA levels to dissect relevant gene products involved in parasite development or destruction coded for, within the relatively large B. glabrata genome.

描述（由申请人提供）：在蜗牛宿主中，有关螺旋体如何生存或针对破坏的基本问题对血吸虫病的流行病学具有重要意义。先进的基础工作清楚地表明，蜗牛生物掌lia glabrata的遗传变异在寄生虫心脏病的内部发育中起主要作用。被遗传选择为抗寄生虫或易感性的蜗牛股为我们提供了有价值的资源来识别标记以及蜗牛/寄生虫关系中重要的基因。我们的目标是使用良好的分子工具，研究具有稳定但易感性表型不同的蜗牛库存，以识别和表征那些在抵抗或完全支持螺旋体感染中起着至关重要作用的蜗牛基因。抗性与易感蜗牛DNA中的多态性应使我们能够有效地分离针对蜗牛/寄生虫关系涉及的序列的探针。还将使用在后代蜗牛中检查多态标记物的遗传力，这些蜗牛具有抗药性或敏感性。通过从暴露的抗性和易感的少年蜗牛中产生抑制减法杂交（SSH）库，并通过筛查可用的蜗牛微阵列，该蜗牛微阵列使用cDNA探测器中的cDNA探针，来自耐药和易感幼年蜗牛（寄生虫感染），我们希望能够识别这些不同感染势型的基因。为了通过鱼类染色体对毛刺芽孢杆菌的位置映射，我们将使用（作为探针）袋克隆，对应于从微阵列/减法研究中确定的转录本。我们的整体策略是减少重复标记的流入，并结合在相对较大的glabrata基因组中，在DNA和RNA水平上获得的有用信息，以剖析与寄生虫发育或破坏有关的相关基因产物。