Core--Microsurgery

核心--显微外科

• 批准号: 7924683
• 负责人: George Tellides
• 金额: $ 21.53万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7924683
• 关键词: Adoptive Transfer; Animals; Aorta; Arterial Injury; Arteries; Arteriosclerosis; Biology; Blood Vessels; Cells; Chimera organism; Chronic; Complex; Coronary artery; Data; Ensure; Experimental Models; Genetic Models; Human; Human Resources; Immunodeficient Mouse; Interferon Type II; Leukocytes; Methodology; Methods; Microsurgery; Modeling; Mus; Play; Quality Control; Rag1 Mouse; Rattus; Research Personnel; Resources; Role; Techniques; Training; Transplantation; Universities; Vascular Graft; cytokine; experience; human tissue; improved; in vivo Model; mutant; programs; reconstitution; skills

The Microsurgery Core will provide expertise and quality control over animal transplantation models for Projects by Tellides, Pober, Min, and Bender of the program. The core unit will serve as a central resource to utilize the human artery transplantation models in immunodeficient mouse chimeras that have been developed at Yale University. Double-mutant severe combined immunodeficient (SCID)/beige mice are grafted with human or synthetic arteries and are subsequently immunologically reconstituted with an adoptive transfer of human leukocytes and/or are treated with human cytokines, such as the species-specific Th1 factor, interferon-gamma (IFN-y). The interactions of the leukocytes or cytokines with the graft vascular cells results in immunemediated arterial injury in a surrogate human experimental model. Additionally, various mouse recipient strains are grafted with mouse aorta segments to take advantage of the power of murine genetic models to supplement the human tissue data. The Microsurgery Core will also develop and adapt the artery graft models according to Project requirements, e.g. developing a Rag1 mutant rat recipient to study remodeling in larger primary branches of human epicardial coronary arteries. All four projects will be supported by the Microsurgery Core. The aims of the Microsurgery Core are:1) to provide the complex small animal transplantation models to the Program investigators; 2) to develop new methods for improving or adapting the in vivo models; and 3) to provide a microsurgery training resource for investigators in vascular and transplantation biology. The methodology of the human artery-SCID/beige mouse transplantation model is relatively complex and requires shared facilities and special skills. The Microsurgery Core personnel have extensive experience with the required techniques and their combined expertise is essential to ensure consistency of the models and an economy of scale. By providing the artery graft models to all of the projects, the Microsurgery Core will play a key role in this program.

显微外科核心将为动物移植模型提供专业知识和质量控制 该计划的 Tellides、Pober、Min 和 Bender 的项目。该核心单元将作为中央资源，利用耶鲁大学开发的免疫缺陷小鼠嵌合体中的人类动脉移植模型 大学。双突变严重联合免疫缺陷（SCID）/米色小鼠被移植人类或 合成动脉，随后通过人的过继转移进行免疫重建 白细胞和/或用人类细胞因子处理，例如物种特异性 Th1 因子、干扰素-γ (干扰素-y)。白细胞或细胞因子与移植血管细胞的相互作用导致免疫介导的 替代人体实验模型中的动脉损伤。此外，各种鼠标接收器 将菌株与小鼠主动脉片段移植，利用小鼠遗传模型的力量 补充人体组织数据。显微外科核心还将开发和改造动脉移植物 根据项目要求的模型，例如开发 Rag1 突变大鼠受体来研究重塑 在人类心外膜冠状动脉的较大初级分支中。所有四个项目都将得到以下机构的支持 显微外科核心。显微外科核心的目标是：1）提供复杂的小动物 向项目研究者移植模型； 2）开发改进或适应的新方法 体内模型； 3) 为血管和外科领域的研究人员提供显微外科培训资源 移植生物学。人动脉-SCID/米色小鼠移植模型的方法是 相对复杂，需要共享设施和特殊技能。显微外科核心人员有 在所需技术方面的丰富经验及其综合专业知识对于确保 模型的一致性和规模经济。通过向所有患者提供动脉移植模型 项目中，显微外科核心将在该计划中发挥关键作用。