Real Time PCR

实时荧光定量PCR

• 批准号: 7630219
• 负责人: Michael C. Ostrowski
• 金额: $ 6.07万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7630219
• 关键词: Agar Gel Electrophoresis; Algorithms; Applications Grants; Archives; Award; Base Sequence; Biological Assay; Budgets; Calendar; Categories; Charge; Chemistry; Class; Code; Complementary DNA; Complex; Computer software; Computers; Consultations; Core Facility; Cost Sharing; DNA; DNA Binding; Daily; Data; Data Analyses; Data Files; Databases; Detection; Development; Discrimination; Dyes; Educational Status; Educational workshop; Electronic Mail; Ensure; Europe; Exons; Facility Designs; Floor; Fluorescence; Funding; Future; Genbank; Gene Amplification; Gene Dosage; Gene Expression; Genes; Goals; Grant; Grant Review; Guidelines; Hour; Housing; Human Resources; Internet; Introns; Lasers; Lead; Learning; Length; Link; Literature; Maintenance; Malignant Neoplasms; Manuals; Measures; Medical; Medical center; Methodology; Methods; Mission; Nucleic acid sequencing; Numbers; Optics; Pediatric Hospitals; Phase; Pliability; Policies; Polymerase Chain Reaction; Polymorphism Analysis; Postdoctoral Fellow; Preparation; Price; Procedures; Process; Production; Productivity; PubMed; Publications; Purpose; Quality Control; RNA Interference; RNA Sequences; Range; Reaction; Reagent; Recommendation; Relative (related person); Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Reverse Transcriptase Polymerase Chain Reaction; Ribosomal RNA; Running; Sales; Sampling; Schedule; Secure; Sequence Analysis; Services; Signal Transduction; Single Nucleotide Polymorphism; Site; Specificity; Standards of Weights and Measures; System; Techniques; Technology; Telephone; Testing; The Comprehensive Cancer Center Arthur G James Cancer Hospital and Richard J Solove Research Institute at Ohio State University; Time; Tissue Sample; Training; Tube; Update; Validation; Vendor; Viral Load result; anticancer research; base; biomedical informatics; cost; day; design; ds-DNA; experience; file format; instrument; instrumentation; interest; member; nuclease; programs; research facility; theories; usability

The mission of the OSUCCC Real Time PCR Shared Resource (RT PCR SR) is to advance the quality of cancer research while increasing productivity of OSUCCC members at reduced costs through providing high quality instrumentation and expert resources. Quantitative real time polymerase chain reaction (QRT-PCR) provides a rapid and accurate method for determination of levels of specific DNA and RNA sequences from tissue samples. It is based on detection of a fluorescent signal produced proportionately during amplification of a PCR product. However, current technology is associated with high costs for reagents, instrumentation, setup, erroneous design/data interpretation and maintenance. For this reason, QRT-PCR expertise and instrumentation are made available to OSUCCC investigators as well as to OSU researchers at large through the RT PCR. The Applied Biosystem's Prism 7700 instrument and expert personnel are located in the Tzagournis Medical Research Facility (TMRF) that is at the center of the OSU Medical Center complex. Over the past two years since this was transitioned from a developmental to a full SR, 52 members, representing 6 of the 60SUCCC Programs, have used the resource, making up >82% of the overall RT PCR usage. To meet the needs of and to ensure the RT PCR SR is accessible to all OSUCCC members, nine services in addition to instrument self-service and training have been added: consultations, primer/probe design, technicianperformed service, aid in developing grants pertaining to Real Time PCR, a website for convenient on-line scheduling and a database of optimized Real Time PCR primers, data analysis/interpretation, data archiving and an in-house store of common reagents and disposables. Based on grants funded in the last year, grants in review, and grants to be submitted in the next year, the current projection in usage of the RT PCR SR's main facility by OSUCCC members is an increase of greater than 2.5 times the current usage. To meet and exceed the RT PCR SR's main goals to increase productivity in a cost-effective manner and advance the quality of cancer research by OSUCCC members, some cost sharing for instrumentation upgrades and new services are proposed.

OSUCCC实时PCR共享资源（RT PCR SR）的任务是提高质量 癌症研究同时提高OSUCCC成员的生产率，从而通过提供高昂的成本来降低成本 优质的仪器和专家资源。定量实时聚合酶链反应（QRT-PCR） 提供了一种快速准确的方法来确定特定DNA和RNA序列的水平 组织样品。它基于在扩增过程中比例产生的荧光信号的检测 PCR产品。但是，当前的技术与试剂，仪器，仪器，仪器的高成本有关 设置，错误的设计/数据解释和维护。因此，QRT-PCR专业知识和 OSUCCC调查人员以及OSU的研究人员都可以使用仪器 RT PCR。应用生物系统的Prism 7700仪器和专家人员位于 Tzagournis医学研究机构（TMRF）位于OSU医疗中心综合大楼的中心。超过 自此以来的过去两年从发育中过渡到完整的SR，52名成员，代表6 在60SUCCC计划中，使用了资源，占总RT PCR使用量的82％。见面 所有OSUCCC成员都可以访问RT PCR SR的需求并确保9个服务。 为工具提供了自助服务和培训：咨询，底漆/探针设计，技术人员pperformform 服务，帮助开发与实时PCR有关的赠款，这是一个网站，可在线方便 调度和优化实时PCR引物的数据库，数据分析/解释，数据归档 以及内部的常见试剂和一次性物品。基于去年资助的赠款，赠款 在审查中，以及将于明年提交的赠款，当前使用RT PCR SR的预测 OSUCCC成员的主要设施的增加大于当前使用情况的2.5倍。见面和 超过RT PCR SR以成本效益的方式提高生产率的主要目标，并提高 OSUCCC成员的癌症研究质量，一些仪器升级的成本分配和新的 提出了服务。