IMAGING APPROACHES FOR DIATOM SILICA CELL WALL SYNTHESIS

硅藻土细胞壁合成的成像方法

• 批准号: 7722436
• 负责人: MARK HILDEBRAND
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722436
• 关键词: Cell Wall; Computer Retrieval of Information on Scientific Projects Database; Diatoms; Electrons; Fluorescence Microscopy; Funding; Goals; Grant; Image; Institution; Location; Monitor; Phytoplankton; Process; Proteins; Research; Research Personnel; Resources; Silicon Dioxide; Source; Structure; Subcellular structure; United States National Institutes of Health; nanostructured

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the process of cell wall synthesis in diatoms, unicellular eukaryotic phytoplankton that make cell walls of nanostructured silica. Electron and fluorescence microscopy is being applied to monitor subcellular structures and their dynamics during cell wall synthesis. In addition, the intracellular location of specific proteins in relation to silica structures is being determined, with a goal of correlating formation of specific structures with the proteins involved.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们正在研究硅藻（单细胞真核浮游植物）细胞壁的合成过程，硅藻可以制造纳米结构二氧化硅的细胞壁。 电子和荧光显微镜被应用于监测细胞壁合成过程中的亚细胞结构及其动态。 此外，正在确定与二氧化硅结构相关的特定蛋白质的细胞内位置，目标是将特定结构的形成与所涉及的蛋白质关联起来。