STRUCTURAL ANALYSIS OF THE RIBOSOME

核糖体的结构分析

• 批准号: 7723600
• 负责人: LEONARDO G TRABUCO
• 金额: $ 6.32万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723600
• 关键词: Amino Acids; Antibiotics; Anticodon; Codon Nucleotides; Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; Funding; Genetic; Grant; Image; Institution; Instruction; Ions; Messenger RNA; Object Attachment; Peptides; Proteins; Research; Research Personnel; Resolution; Resources; Ribosomal Proteins; Ribosomal RNA; Ribosomes; Source; Translations; United States National Institutes of Health; X-Ray Crystallography; base; insight; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ribosome [1] is a cellular machine that synthesizes proteins based on genetic instructions. The ribosome moves along the mRNA, catches tRNAs, facilitates the pairing between codons and anticodons, and catalyzes the formation of peptide bonds between amino acids. The bacterial ribosome is an important target of antibiotics; indeed, 50% of all research on antibiotics is focused on the ribosome. Currently the most successful approaches to image ribosomes are cryo-electron microscopy (cryo-EM) [2] and X-ray crystallography [3]. Cryo-EM offers insights into the function of the ribosome by providing snapshots of different functional states, currently at a resolution of 7 Angstroms. X-ray crystallography yields atomic-scale structural information for single or undefined functional states. These and other experiments show that the ribosome consists of two subunits, the small subunit being responsible for codon-anticodon recognition, and the large subunit for catalyzing peptide bond formation. The whole translation machinery consists of ribosomal RNAs, about 50 ribosomal proteins, tRNAs, mRNA, ions, and additional protein factors.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 核糖体[1]是一种基于遗传指示来合成蛋白质的细胞机。 核糖体沿mRNA移动，捕获TRNA，促进密码子和抗密码子之间的配对，并催化氨基酸之间肽键的形成。 细菌核糖体是抗生素的重要靶标。实际上，所有关于抗生素研究的研究中有50％集中在核糖体上。 目前，最成功的图像核糖体方法是冷冻电子显微镜（Cryo-EM）[2]和X射线晶体学[3]。 Cryo-Em通过提供不同功能状态的快照，目前以7埃埃斯特罗姆的分辨率提供了核糖体功能的见解。 X射线晶体学产生单个或不确定功能状态的原子尺度结构信息。 这些和其他实验表明，核糖体由两个亚基组成，小亚基负责密码子 - 抗原识别，以及用于催化肽键形成的大型亚基。 整个翻译机械由核糖体RNA组成，大约50个核糖体蛋白，TRNA，mRNA，离子和其他蛋白质因子。