TRACE ANESTHETIC & METABOLITE IN CHIRAL & RACEMIC MIXTURES OF SECONDARY ALCOHOLS

微量麻醉剂

• 批准号: 7724201
• 负责人: JAMES Michael SONNER
• 金额: $ 1.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724201
• 关键词: Alcohols; Anesthetics; Animals; Computer Retrieval of Information on Scientific Projects Database; Funding; Gas Chromatography; Grant; Institution; Ion Channel; Mass Spectrum Analysis; Morphologic artifacts; Pattern; Research; Research Personnel; Resources; Source; Suggestion; United States National Institutes of Health; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 2-alcohols are anesthetics. We have been studying the anesthetic effects of these compounds in animals and on expressed ion channels. We have observed an unusual potency pattern for these compounds both in animals and on receptors suggestion that an impurity might be present. Although these compounds are purchased in approximately 99% purity, a trace compound that is volatile and very lipophilic may be present and exerting significant anesthetic effects. Several trace compounds are observed by gas chromatography. We need mass spectrometry to determine what these trace compounds are in order to evaluate whether they have actions as anesthetics. This will permit us to know whether the observed potency relations are real, which would have mechanistic significance, or an artifact caused by an impurity. In addition, a metabolite appears to be produced in animals and this too requires identification, in order to correct the potency of the secondary alcohol for the presence of this compound.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 2-醇是麻醉药。 我们一直在研究这些化合物在动物和表达离子通道中的麻醉作用。 我们已经观察到这些化合物在动物和受体中的异常效能模式，暗示可能存在杂质。 尽管这些化合物的纯度约为99％，但可能存在易碎性且非常亲脂性的痕量化合物并发挥明显的麻醉作用。 通过气相色谱观察几种痕量化合物。 我们需要质谱法来确定这些痕量化合物是什么，以评估它们是否具有麻醉作用。 这将使我们知道观察到的效力关系是真实的，它具有机械意义，还是由杂质引起的人工制品。 此外，为了纠正该化合物存在的二次酒精的效力，似乎在动物中产生了一种代谢物，这也需要鉴定。