MOUSE METABOLIC PHENOTYPING CENTER

小鼠代谢表型中心

• 批准号: 7724108
• 负责人: Craig R Malloy
• 金额: $ 5.23万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724108
• 关键词: Animal Model; Animals; Basic Science; Biological Assay; Cations; Citric Acid Cycle; Computer Retrieval of Information on Scientific Projects Database; Computers; Data; Detection; Disease; Fatty acid glycerol esters; Funding; Gluconeogenesis; Glucose; Glycerol; Grant; Hepatic; Institution; Internet; Life; Liver; Measures; Metabolic; Metabolism; Methods; Modeling; Mus; NMR Spectroscopy; Non-Insulin-Dependent Diabetes Mellitus; Phenotype; Physicians; Protocols documentation; Pyruvate; Pyruvates; Recycling; Research; Research Personnel; Resources; Scientist; Series; Site; Source; Standards of Weights and Measures; Techniques; Testing; Tracer; United States National Institutes of Health; design; glucose production; improved; in vivo; oxidation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this project is to establish standard mouse phenotyping techniques to quantitatively evaluate liver gluconeogenesis, pyruvate recycling, the contribution of glycerol and Krebs cycle intermediates to glucose production, total glucose turnover, and citric acid cycle flux in live animals using a combination of 13C and 2H tracers and NMR spectroscopy. This standard metabolic assay provides the most comprehensive existing analysis of hepatic intermediary metabolism. It will be made available to on-campus and off-campus investigators having animal models of NIDDM or related disorders. In addition to the standard metabolic profile described above, a series of other phenotype specific metabolic techniques including a quantitative measure of substrate oxidation, measures of intracellular cations, and citric acid cycle flux using 13C enriched substrates will also be made available for selected animal models. A unique feature of this phenotyping center is that input will come from chemists, physicists, physiologists, computer scientists, physicians, and geneticists. All are recognized experts in 13C isotopomer analysis. A 600 MHz NMR spectrometer will be dedicated to this project. All mice selected for phenotyping will be subjected to a standard protocol performed by high-level technicians; the data will be analyzed and interpreted by NMR spectroscopists and biochemists and made available through an interactive web site designed to allow the external investigators to test a fit of their isotopomer data to alternative metabolic models. A basic research core will focus on improving sensitivity through indirect detection methods and develop methods to measure fat oxidation in vivo. Finally, an administrative core will coordinate the activities of the three research cores and interface discussions between external investigators and center scientists.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目的目的是建立标准的小鼠表型技术，以定量评估肝糖生成，丙酮酸回收，甘油和克雷布斯循环中间体对葡萄糖产生，总葡萄糖更换和柠檬酸周期的贡献，并使用13C和2H Tracracers和2H Tracers and Nmrspemproscopy组合在现场动物中。该标准的代谢测定法提供了对肝中介代谢的最全面的现有分析。它将提供给具有NIDDM或相关疾病动物模型的校园和校外调查人员。除了上述标准代谢谱外，还将提供一系列其他表型的特异性代谢技术，包括底物氧化的定量度量，细胞内阳离子的测量以及使用13C富集的底物的柠檬酸循环通量的测量，也将用于选定的动物模型。该表型中心的一个独特特征是，输入将来自化学家，物理学家，生理学家，计算机科学家，医师和遗传学家。所有人都是13C同位素分析中公认的专家。 600 MHz NMR光谱仪将专门用于该项目。所有选择表型的小鼠将遵守高级技术人员执行的标准方案； NMR光谱学家和生物化学家将对数据进行分析和解释，并通过交互式网站提供，旨在允许外部研究者测试其同位素数据对替代代谢模型的拟合。基础研究核心将专注于通过间接检测方法提高灵敏度，并开发在体内测量脂肪氧化的方法。最后，行政核心将协调三个研究核心的活动以及外部研究人员与中心科学家之间的界面讨论。