SERVICE

服务

• 批准号: 7724563
• 负责人: Milos Novotny
• 金额: $ 4万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724563
• 关键词: Cleaved cell; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Methods; Monosaccharides; Peptide Mapping; Peptides; Polysaccharides; Post-Translational Protein Processing; Protein Fingerprints; Proteins; Proteomics; Research; Research Personnel; Resources; Services; Source; Standards of Weights and Measures; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; glycosylation; liquid chromatography mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed Center will provide access to its existing methods as well as newly developed methods through a service component. The analytical services it will provide include a comprehensive set of methods for the analysis of monosaccharides using CE, polysaccharides cleaved from proteins using MS, and glycosylation of tryptic peptides using LC/MS methods. The services offered will also include standard proteomic analyses such as 2D gel electrophoresis, peptide fingerprinting for protein identification, and the analysis of post-translational protein modifications other than glycosylation.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 拟议的中心将通过服务组件提供对其现有方法和新开发方法的访问。它将提供的分析服务包括使用 CE 分析单糖、使用 MS 从蛋白质中裂解多糖以及使用 LC/MS 方法分析胰蛋白酶肽的糖基化的一整套方法。提供的服务还将包括标准蛋白质组分析，例如二维凝胶电泳、用于蛋白质鉴定的肽指纹分析以及除糖基化之外的翻译后蛋白质修饰分析。